1887

Abstract

Three microbial strains isolated from common beans, 23C2 (Tunisia), Gr42 (Spain) and IE4868 (Mexico), which have been identified previously as representing a genomic group closely related to , are further studied here. Their 16S rRNA genes showed 98.5–99 % similarity with CCBAU 7190B, R602sp, USDA 1844 and CCBAU 71623. Phylogenetic analysis based on , , and sequences showed that the novel strains were closely related and could be distinguished from the four type strains of the closely related species. Strains 23C2, Gr42 and IE4868 could be also differentiated from their closest phylogenetic neighbours by their phenotypic and physiological properties and their fatty acid contents. All three strains harboured symbiotic genes specific to biovar Levels of DNA–DNA relatedness between strain 23C2 and the type strains of , and ranged from 58.1 to 61.5 %. The DNA G+C content of the genomic DNA of strain 23C2 was 59.52 %. On the basis of these data, strains 23C2, Gr42 and IE4868 were considered to represent a novel species of the genus for which the name is proposed. Strain 23C2 ( = CCBAU 101087 = HAMBI3541) was designated as the type strain.

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2014-05-01
2019-12-13
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