1887

Abstract

A novel acidobacterial strain, Sakura1, was isolated from pieces of cherry bark. Cells of strain Sakura1 were non-motile, rod-shaped and stained Gram-negative. This strictly aerobic isolate was mesophilic but was able to grow at temperatures as low as 10 °C. Colonies were pink due to production of carotenoids, and its pigmentation was more pronounced in cells grown at lower temperature. This strain had endoglucanase activity. The main respiratory quinone was menaquinone-8, and major cellular fatty acids were iso-C, Cω7 and C. Phylogenetic analysis of 16S rRNA gene sequences revealed that the strain was closely related to species of the genus in subdivision 1 of the phylum . The closest relative was OB1010 (97.1 % 16S rRNA gene sequence similarity). While common characteristics were found among the isolate and species of the genus , there were obvious differences between them such as their cell morphology, cellulolytic activity, and tolerance to low temperature and NaCl concentration. Based on phylogenetic and phenotypic findings, a novel species is proposed in the genus with the name sp. nov. The type strain is Sakura1 ( = NBRC 107139 = DSM 23641).

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2014-08-01
2019-10-15
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