1887

Abstract

An alkaliphilic and halotolerant Gram-stain-positive bacterium, which was isolated from sediment samples from the South China Sea, was subjected to a taxonomic study. The isolate, strain L1, grew well at a wide range of temperatures and pH values, 10.0–45.0 °C and pH 6–11, with optima at 30 °C and pH 9.0, respectively. The growth of strain L1 occurred at total salt concentrations of 0–10 % (w/v) with an optimum at 2 % (w/v). Phylogenetic analysis based on 16S rRNA sequence comparison indicated that the isolate represented a member of the genus . The strains most closely related to strain L1 were JCM 16507, DSM 497 and DSM 8715, with 16S rRNA similarities of 96.5 %, 95.9 % and 95.7 %, respectively. DNA–DNA hybridization of strain L1 with the type strains of the most closely related species, JCM 16507, DSM 497 and DSM 8715, showed reassociation values of about 21.7 %, 14.3 % and 13.9 %, respectively. The DNA G+C content of strain L1 was 40.76 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids of strain L1 were iso-C and anteiso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the phenotypic and phylogenetic characteristics, it is proposed that strain L1 ( = JCM 18543 = DSM 26145) should be classified as the type strain of sp. nov.

Funding
This study was supported by the:
  • Natural Science Foundation of Jiangsu Province (Award BK2012695)
  • Technology Innovation Fund for Small and Medium-sized Enterprises of Jiangsu Province (Award BK2012695)
  • State Key Laboratory of Dairy Biotechnology (Award SKLDB2011-003)
  • Scientific Research Foundation for the Returned Overseas Chinese Scholars
  • State Education Ministry
  • Priority Academic Program Development of Jiangsu Higher Education Institutions
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2014-05-01
2024-04-19
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References

  1. Amoozegar M. A., Sánchez-Porro C., Rohban R., Hajighasemi M., Ventosa A. ( 2009 ). Bacillus persepolensis sp. nov., a moderately halophilic bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 59, 23522358. [View Article] [PubMed]
    [Google Scholar]
  2. Collins M. D., Jones D. ( 1981 ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45, 316354.[PubMed]
    [Google Scholar]
  3. Cowan S. T. ( 1974 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 2nd edn.. Cambridge:: Cambridge University Press;.
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  6. Gerhardt P. ( 1994 ). Methods for General and Molecular Bacteriology. Washington, D.C.:: American Society for Microbiology;.
    [Google Scholar]
  7. Goodfellow M., Minnikin D. E. ( 1985 ). Chemical Methods in Bacterial Systematics. London:: Academic;.
    [Google Scholar]
  8. Gugliandolo C., Maugeri T. L., Caccamo D., Stackebrandt E. ( 2003 ). Bacillus aeolius sp. nov. a novel thermophilic, halophilic marine Bacillus species from Eolian Islands (Italy). . Syst Appl Microbiol 26, 172176. [View Article] [PubMed]
    [Google Scholar]
  9. Humane R. E. ( 1969 ). A roll tube method for cultivation of strict anaerobes. . Methods Microbiol 3B, 117132.
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. ( 2004 ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5, 150163. [View Article] [PubMed]
    [Google Scholar]
  11. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [View Article] [PubMed]
    [Google Scholar]
  12. Nielsen P., Fritze D., Priest F. G. ( 1995 ). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. . Microbiology 141, 17451761. [View Article]
    [Google Scholar]
  13. Reddy C. A. (editor) ( 2007 ). Methods for General and Molecular Microbiology, , 3rd edn.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  14. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  15. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  16. Shi R., Yin M., Tang S. K., Lee J. C., Park D. J., Zhang Y. J., Kim C. J., Li W. J. ( 2011 ). Bacillus luteolus sp. nov., a halotolerant bacterium isolated from a salt field. . Int J Syst Evol Microbiol 61, 13441349. [View Article] [PubMed]
    [Google Scholar]
  17. Sorokin D. Y., van Pelt S., Tourova T. P. ( 2008 ). Utilization of aliphatic nitriles under haloalkaline conditions by Bacillus alkalinitrilicus sp. nov. isolated from soda solonchak soil. . FEMS Microbiol Lett 288, 235240. [View Article] [PubMed]
    [Google Scholar]
  18. Springer N., Ludwig W., Amann R., Schmidt H. J., Görtz H. D., Schleifer K. H. ( 1993 ). Occurrence of fragmented 16S rRNA in an obligate bacterial endosymbiont of Paramecium caudatum . . Proc Natl Acad Sci U S A 90, 98929895. [View Article] [PubMed]
    [Google Scholar]
  19. Takami H., Krulwich T. A. ( 2000 ). Reidentification of facultatively alkaliphilic Bacillus firmus OF4 as Bacillus pseudofirmus OF4. . Extremophiles 4, 1922. [View Article] [PubMed]
    [Google Scholar]
  20. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  21. Tindall B. J. ( 1990 ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [View Article]
    [Google Scholar]
  22. Woese C. R., Gutell R., Gupta R., Noller H. F. ( 1983 ). Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. . Microbiol Rev 47, 621669.[PubMed]
    [Google Scholar]
  23. Yumoto I., Yamaga S., Sogabe Y., Nodasaka Y., Matsuyama H., Nakajima K., Suemori A. ( 2003 ). Bacillus krulwichiae sp. nov., a halotolerant obligate alkaliphile that utilizes benzoate and m-hydroxybenzoate. . Int J Syst Evol Microbiol 53, 15311536. [View Article] [PubMed]
    [Google Scholar]
  24. Zhang J., Wang J., Song F., Fang C., Xin Y., Zhang Y. ( 2011a ). Bacillus nanhaiisediminis sp. nov., an alkalitolerant member of Bacillus rRNA group 6. . Int J Syst Evol Microbiol 61, 10781083. [View Article] [PubMed]
    [Google Scholar]
  25. Zhang L., Wu G. L., Wang Y., Dai J., Fang C. X. ( 2011b ). Bacillus deserti sp. nov., a novel bacterium isolated from the desert of Xinjiang, China. . Antonie van Leeuwenhoek 99, 221229. [View Article] [PubMed]
    [Google Scholar]
  26. Zhu D., Li P., Tanabe S. H., Sun J. ( 2013 ). Genome sequence of the alkaliphilic bacterial strain Bacillus ligninesis L1, a novel degrader of lignin. . Genome Announc 1, e0004213. [View Article] [PubMed]
    [Google Scholar]
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