sp. nov., isolated from root nodules Free

Abstract

Three strains of Gram-stain-negative, rod-shaped bacteria were isolated from root nodules and authenticated on this host. Based on the 16S rRNA gene sequence phylogeny, they were shown to belong to the genus , with the representative strain WSM3556 being most closely related to LMG 23644 (98.70 % 16S rRNA gene sequence similarity) and WSM3937 (98.50 %). Additionally, these strains formed a distinct group in phylogenetic trees of the housekeeping genes and . Chemotaxonomic data, including fatty acid profiles and analysis of respiratory quinones, supported the assignment of our strains to the genus . Results of DNA–DNA hybridizations, MALDI-TOF MS analysis and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from their nearest neighbour species. Therefore, these strains represent a novel species, for which the name sp. nov. is proposed, with the type strain WSM3556 ( = LMG 27173 = HAMBI 3353).

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2014-04-01
2024-03-29
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