1887

Abstract

A novel Gram-staining-positive, moderately halophilic bacterium, designated strain A76, was isolated from a brine sample of the hypersaline lake Aran-Bidgol in Iran. Cells were strictly aerobic, coccus-shaped, non-motile, non-sporulating, and catalase- and oxidase-positive. Strain A76 grew between pH 7.0 and 10.0 (optimal growth at pH 8.0), between 20 and 45 °C (optimal growth at 35 °C) and at salinities of 0.5 to 12.5 % (w/v) NaCl (optimal growth at 7.5 %, w/v, NaCl). On the basis of 16S rRNA gene sequence analysis, strain A76 was shown to belong to the phylum with sequence similarities of 94.1, 93.1 and 91.1 %, to the type species of the genera , and , respectively. The DNA G+C content of this new isolate was 38.8 mol%. The major cellular fatty acids of strain A76 were anteiso-C and iso-C, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, an unknown lipid and two unknown phospholipids. The isoprenoid quinones were MK-6 (94 %), MK-5 (3 %) and MK-7 (3 %). The amino acid constituents of the cell wall were Lys, Asp, Gly, Glu and Ala. The physiological, biochemical and phylogenetic differences between strain A76 and type strains of taxa with validly published names suggest that this strain represents a novel species in a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is strain A76 ( = CECT 8508 = DSM 28306 = IBRC-M 10081).

Funding
This study was supported by the:
  • Research Council, University of Tehran
  • Iranian Biological Resource Centre (IBRC) (Award MI-1388-04)
  • Spanish Ministerio de Ciencia e Innovación (Award CGL2010-19303)
  • National Science Foundation (Award DEB-0919290)
  • Junta de Andalucía (Award P10-CVI-6226)
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2014-06-01
2022-01-28
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