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Abstract

A Gram-stain-positive, strictly aerobic, coccus-shaped, non-motile, yellow-pigmented bacterium, designated strain XH208, was isolated from a deep subseafloor sediment sample collected from the South Pacific Gyre (41° 58′ S 163° 11′ W) during the Integrated Ocean Drilling Program (IODP) Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XH208 belonged to the genus and showed the highest 16S rRNA gene sequence similarities with CCUG 38120 (96.9 %), DSM 10546 (95.4 %) and CCUG 33897 (95.2 %). The DNA G+C content of strain XH208 was 66.9 mol%. The cell wall of strain XH208 possessed a type A3γ peptidoglycan (-diaminopimelic acid–glycine), and ribose, glucose and galactose as the major whole-cell sugars. The major fatty acids were Cω8, Cω6, and Cω6 and/or Cω7 (summed feature 3). The major respiratory quinone was menaquinone MK-9(H). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol. On the basis of data from the polyphasic analysis, strain XH208 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is XH208 ( = DSM 27277 = JCM 19259).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41276141)
  • National High Technology Research & Development Program of China (Award 2012AA091605)
  • China Ocean Mineral Resources Research & Development Association (Award DY125-15-R-03)
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2014-08-01
2024-03-29
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