1887

Abstract

A neutrophilic, stalk-forming, iron-oxidizing bacterium, strain OYT1, which was isolated from a groundwater seep in Ohyato Park, Tokyo, Japan, was subjected to taxonomic analysis. OYT1 was a motile, bean-shaped, Gram-negative bacterium that was able to grow at 8–30 °C (optimally at 25–30 °C) and at pH 5.6–7.3 (optimally at pH 6.1–6.5). The strain grew microaerobically and autotrophically. Major cellular fatty acids detected were Cω7/Cω6 and C. The total DNA G+C content was 57.6 mol%. 16S rRNA gene sequence analysis revealed that strain OYT1 was affiliated with the class and clustered with iron-oxidizing bacteria isolated from groundwater seeps and wetlands and with uncultured clones detected in freshwater iron-rich environments. Based on the phenotypic and phylogenetic characteristics of strain OYT1, we propose that the strain represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain of is OYT1 ( = JCM 18545 = DSM 26810).

Funding
This study was supported by the:
  • , RIKEN Special Postdoctoral Researchers’ Program
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2014-03-01
2020-08-09
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