1887

Abstract

A Gram-staining-negative, strictly aerobic, white-colony-forming bacterium, designated strain 5-10, was isolated from forest soil of Bac Kan Province in Vietnam. Cells were non-motile rods or coccoids, showing oxidase- and catalase-positive reactions. Growth was observed at 10–37 °C (optimum, 30 °C), at pH 5.0–9.0 (optimum, pH 7.0) and in the presence of 0–1.0 % (w/v) NaCl (optimum, 0–0.5 %). The major cellular fatty acids were summed feature 3 (comprising Cω6 and/or Cω7), C, C 3-OH and summed feature 8 (comprising Cω6 and/or Cω7). The G+C content of the genomic DNA was 69.9 mol% and the only respiratory quinone detected was ubiquinone 8 (Q-8). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 5-10 formed a tight phyletic lineage with members of the genus . Strain 5-10 was most closely related to TTB310 (97.3 %), but the DNA–DNA relatedness level between the two strains was 38.2±1.8 %. Based on phenotypic, chemotaxonomic and molecular features, strain 5-10 was shown to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5-10 ( = KACC 17567 = JCM 19319). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • , National Institute of Biological Resources (NIBR) of the Ministry of Environment , (Award 2012-02-044)
  • , Technology Development Program for Agriculture and Forestry (TDPAF) of the Ministry for Agriculture, Food and Rural Affairs, Republic of Korea
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2014-04-01
2020-05-29
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