1887

Abstract

A novel Gram-staining-positive, aerobic bacterium, designed DCY80, was isolated from soil of a ginseng field in the Republic of Korea. 16S rRNA gene sequence analysis revealed that strain DCY80 belonged to the genus (95.8–98.2 % similarity) and was most closely related to DSM 4810 (98.2 %). Colonies were circular, entire, low-convex, opaque and 0.5–1.0 mm in diameter after growth for 2 days on TSA at 30 °C. Growth occurred at 4–34 °C (optimum, 25 °C), at pH 5.0–10.0 (optimum, pH 6.5–7.0) and in the presence of 0–7.0 % NaCl. Strain DCY80 produced siderophores and was sensitive to penicillin G, erythromycin, cefazolin, oleandomycin, ceftazidime, vancomycin, tetracycline, novobiocin, carbamicillin, rifampicin and neomycin. The DNA G+C content was 71.0 mol%. Levels of DNA–DNA relatedness between strain DCY80 and DSM 4810, KCTC 9916, DSM 23186 and KCTC 9915 were 46.9±0.5, 28.9±0.6, 20.4±0.9 and 17.3±0.4 %, respectively. The cell-wall peptidoglycan of strain DCY80 contained -diaminopimelic acid as the diagnostic diamino acid. The menaquinones were MK-7 (85.8 %) and MK-8 (14.2 %). The major cellular fatty acids were anteiso-C (69.1 %) and anteiso-C (12.2 %). Phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid, two unidentified phospholipids and five unidentified polar lipids were found. On the basis of our phenotypic and genotypic analyses, strain DCY80 represents a novel species of the genus , for which the name sp. nov. is proposed (type strain DCY80 = KCTC 29226 = JCM 19356).

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2014-09-01
2019-11-18
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