1887

Abstract

Bacterial strains KIS82-1 and GRS42 were isolated from soil and from sap of , respectively, in the Republic of Korea. Both strains were aerobic, Gram-stain-positive, mesophilic, rod-shaped and motile. Phylogenetically, both strains belonged to the family of the phylum . The 16S rRNA gene sequence of strain KIS82-1 showed the highest similarity to those of RS-15 (97.6 %), MSL-08 (97.2 %) and KSW2-17 (97.0 %), while strain GRS42 showed the highest 16S rRNA gene sequence similarity to RS-15 (98.7 %), CafT13 (98.4 %), E1HC-02 (98.2 %) and 801 (97.3 %). The 16S rRNA gene sequence similarity between GRS42 and KIS82-1 was 97.0 %. Phylogenetic trees indicated that strain GRS42 was firmly grouped into the genus , while strain KIS82-1 did not show a clear affiliation to any genus within the family . Strain KIS82-1 showed type B1β peptidoglycan with 2,4-diamino--butyric acid as the diamino acid. It had MK-11, MK-10 and MK-12 as respiratory quinones, anteiso-C, iso-C and iso-C as major cellular fatty acids and diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid as predominant polar lipids. The peptidoglycan of strain GRS42 was of type B2β with -ornithine as the diamino acid. The strain contained MK-8, MK-9 and MK-7 as respiratory quinones, summed feature 8 (Cω6 and/or Cω7) as major cellular fatty acid and diphosphatidylglycerol, phosphatidylglycerol and three unknown glycolipids as predominant polar lipids. Strain GRS42 revealed low DNA–DNA hybridization (<50 % relatedness) with closely related strains. Based on the data obtained in the present polyphasic taxonomic study, we propose that strain KIS82-1 represents a novel genus and species and that strain GRS42 represents a novel species in the family . The genus gen. nov. is proposed, with strain KIS82-1 ( = KACC 15520 = NBRC 108727) as the type strain of the type species, sp. nov. Strain GRS42 ( = KACC 15521 = NBRC 108728) is proposed as the type strain of sp. nov.

Funding
This study was supported by the:
  • , National Academy of Agricultural Science, Rural Development Administration, Republic of Korea , (Award PJ008666)
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2014-02-01
2020-08-14
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