1887

Abstract

A polyvinyl-alcohol-degrading marine bacterium was isolated from plastic rope litter found in Tokyo Bay, Japan. The isolated strain, Zumi 95, was a Gram-reaction-negative, non-spore-forming and facultatively anaerobic chemo-organotroph. The major respiratory quinone was Q-10. The predominant fatty acids were Cω7 and C. On the basis of 16S rRNA gene sequence analysis, the isolated strain was closely affiliated with members of the genus in the class . The DNA G+C content of the novel strain was 55.1 mol%. The hybridization values for DNA–DNA relatedness between this strain and four reference strains representing species of the genus were significantly lower than that accepted as the phylogenetic definition of a species. On the basis of differences in taxonomic characteristics, the isolated strain represents a novel species of the genus for which the name sp. nov. (type strain Zumi 95 = JCM 18746 = DSM 26719) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.058321-0
2014-04-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/4/1149.html?itemId=/content/journal/ijsem/10.1099/ijs.0.058321-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. New York:: Cambridge University Press;. [View Article]
    [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R. D. ( 1972 ). Taxonomy of aerobic marine eubacteria. . J Bacteriol 110, 402429.[PubMed]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  5. Finley J. H. ( 1961 ). Spectrophotometric determination of polyvinyl alcohol in paper coatings. . Anal Chem 33, 19251927. [View Article]
    [Google Scholar]
  6. Hu X., Mamoto R., Shimomura Y., Kimbara K., Kawai F. ( 2007 ). Cell surface structure enhancing uptake of polyvinyl alcohol (PVA) is induced by PVA in the PVA-utilizing Sphingopyxis sp. strain 113P3. . Arch Microbiol 188, 235241. [View Article] [PubMed]
    [Google Scholar]
  7. Hugh R., Leifson E. ( 1953 ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. . J Bacteriol 66, 2426.[PubMed]
    [Google Scholar]
  8. Kawai F., Hu X. ( 2009 ). Biochemistry of microbial polyvinyl alcohol degradation. . Appl Microbiol Biotechnol 84, 227237. [View Article] [PubMed]
    [Google Scholar]
  9. Kodama Y., Stiknowati L. I., Ueki A., Ueki K., Watanabe K. ( 2008 ). Thalassospira tepidiphila sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from seawater. . Int J Syst Evol Microbiol 58, 711715. [View Article] [PubMed]
    [Google Scholar]
  10. Liu C., Wu Y., Li L., Ma Y., Shao Z. ( 2007 ). Thalassospira xiamenensis sp. nov. and Thalassospira profundimaris sp. nov.. Int J Syst Evol Microbiol 57, 316320. [View Article] [PubMed]
    [Google Scholar]
  11. López-López A., Pujalte M. J., Benlloch S., Mata-Roig M., Rosselló-Mora R., Garay E., Rodríguez-Valera F. ( 2002 ). Thalassospira lucentensis gen. nov., sp. nov., a new marine member of the α-Proteobacteria . . Int J Syst Evol Microbiol 52, 12771283. [View Article] [PubMed]
    [Google Scholar]
  12. MIDI ( 1999 ). Sherlock, Microbial Identification System, Operating Manual, version 3.0. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  13. Miyazaki M., Nogi Y., Usami R., Horikoshi K. ( 2006 ). Shewanella surugensis sp. nov., Shewanella kaireitica sp. nov. and Shewanella abyssi sp. nov., isolated from deep-sea sediments of Suruga Bay, Japan. . Int J Syst Evol Microbiol 56, 16071613. [View Article] [PubMed]
    [Google Scholar]
  14. Miyazaki M., Nogi Y., Ohta Y., Hatada Y., Fujiwara Y., Ito S., Horikoshi K. ( 2008 ). Microbulbifer agarilyticus sp. nov. and Microbulbifer thermotolerans sp. nov., agar-degrading bacteria isolated from deep-sea sediment. . Int J Syst Evol Microbiol 58, 11281133. [View Article] [PubMed]
    [Google Scholar]
  15. Saito H., Miura K. I. ( 1963 ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72, 619629. [View Article] [PubMed]
    [Google Scholar]
  16. Stackebrandt E., Ebers J. ( 2006 ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33, 152155.
    [Google Scholar]
  17. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  20. Tokiwa Y., Kawabata G., Jarerat A. ( 2001 ). A modified method for isolating poly(vinyl alcohol)-degrading bacteria and study of their degradation patterns. . Biotechnol Lett 23, 19371941. [View Article]
    [Google Scholar]
  21. Uchida H., Hamana K., Miyazaki M., Yoshida T., Nogi Y. ( 2012 ). Parasphingopyxis lamellibrachiae gen. nov., sp. nov., isolated from a marine annelid worm. . Int J Syst Evol Microbiol 62, 22242228. [View Article] [PubMed]
    [Google Scholar]
  22. Zhao B., Wang H., Mao X., Li R. ( 2009 ). Biodegradation of phenanthrene by a halophilic bacterial consortium under aerobic conditions. . Curr Microbiol 58, 205210. [View Article] [PubMed]
    [Google Scholar]
  23. Zhao B., Wang H., Li R., Mao X. ( 2010 ). Thalassospira xianhensis sp. nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium. . Int J Syst Evol Microbiol 60, 11251129. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.058321-0
Loading
/content/journal/ijsem/10.1099/ijs.0.058321-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error