1887

Abstract

A novel, strictly anaerobic, methylotrophic marine methanogen, strain SLH33, was isolated from deep sediment samples covered by an orange microbial mat collected from the Napoli Mud Volcano. Cells of strain SLH33 were Gram-stain-negative, motile, irregular cocci that occurred singly. Cells utilized trimethylamine, dimethylamine, monomethylamine, methanol, betaine, ,-dimethylethanolamine and choline (,,-trimethylethanolamine) as substrates for growth and methanogenesis. The optimal growth temperature was 30 °C; maximum growth rate was obtained at pH 7.0 in the presence of 0.5 M Na. The DNA G+C content of strain SLH33 was 43.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLH33 within the genus . The novel isolate was related most closely to TMA-10 (98.8 % 16S rRNA gene sequence similarity) but distantly related to DSM 6242 (97.6 %) and AK-5 (97.6 %). DNA–DNA hybridization studies indicated that strain SLH33 represents a novel species, given that it shared less than 16 % DNA–DNA relatedness with TMA-10. The name sp. nov. is proposed for this novel species, with strain SLH33 ( = DSM 26966 = JCM 19278) as the type strain. An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • HERMES (Award GOCECT- 2005-511234-1)
  • HERMIONE (Award 226354)
  • European Union (Award 311975)
  • UBO
  • CNRS
  • Ifremer
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2014-06-01
2024-12-12
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