1887

Abstract

In the present study we investigated the taxonomic status of 20 lactic acid bacteria (LAB) originating from packaged meat. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the genus with , and as the closest phylogenetic relatives. The novel strains shared more than 70 % DNA–DNA relatedness with type and reference strains of both and . The DNA–DNA relatedness values between type and reference strains and type and reference strains were also above 70 %, showing that all these strains belonged to the same species. Sequence analyses of concatenated , , and genes demonstrated that the novel strains as well as type and reference strains of and are phylogenetically closely related, but form three clearly separated subgroups. Numerical analysis of dIII ribopatterns and phenotypic tests supported this subdivision. Based on the data presented in this study, we propose to reclassify as subsp. comb. nov. (type strain, LMG 18811 = DSM 15947). The novel strains isolated in the present study represent a novel subspecies, for which the name subsp. subsp. nov. is proposed, with POUF4d ( = LMG 27840 = DSM 19375) as the type strain. The proposal of these two novel subspecies automatically creates the subspecies subsp. subsp. nov. (type strain, NCFB 2775 = DSM 5578). An emended description of is also provided.

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2014-04-01
2020-01-18
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