1887

International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_4

sp. nov., isolated from the vaginal mucosa of a beef cow, and emended descriptions of , , , , , and Free

Abstract

A gram-stain-positive, facultatively anaerobic, non-spore-forming, catalase-negative, coccoid-shaped bacterial strain, designated BV2, was isolated from the vaginal mucosa of a beef cow in Japan. Phylogenetic analysis showed that the isolate shared high 16S rRNA gene sequence similarity (92.9 %) with 1821/02 and low similarity (<92.7 %) with any other recognized species of the genus . The DNA G+C content was 44.7 mol%, which is within the range observed among species of the genus (37.5–48.4 mol%). The major cellular fatty acid was Cω9, similar to other type strains of species of the genus . The results of genotypic, phenotypic and chemotaxonomic analyses as well as the low degree of DNA–DNA relatedness with all recognized members of the genus indicate that strain BV2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BV2 ( = JCM 19163 = DSM 27293). Emended descriptions of , , , , , and are also presented.

  • Published Online:
Funding
This study was supported by the:
  • , Grant-in-Aid for Young Scientists (A) , (Award 25712032)
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.058081-0
2014-04-01
2021-02-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/4/1229.html?itemId=/content/journal/ijsem/10.1099/ijs.0.058081-0&mimeType=html&fmt=ahah

References

  1. Aguirre M., Collins M. D. ( 1992 ). Phylogenetic analysis of some Aerococcus-like organisms from urinary tract infections: description of Aerococcus urinae sp. nov.. J Gen Microbiol 138, 401–405. [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins M. D., Jovita M. R., Hutson R. A., Ohlén M., Falsen E. ( 1999 ). Aerococcus christensenii sp. nov., from the human vagina. . Int J Syst Bacteriol 49, 1125–1128. [CrossRef] [PubMed]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane-filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [CrossRef]
    [Google Scholar]
  4. Felis G. E., Torriani S., Dellaglio F. ( 2005 ). Reclassification of Pediococcus urinaeequi (ex Mees 1934) Garvie 1988 as Aerococcus urinaeequi comb. nov.. Int J Syst Evol Microbiol 55, 1325–1327. [CrossRef] [PubMed]
    [Google Scholar]
  5. Herthelius M., Gorbach S. L., Möllby R., Nord C. E., Pettersson L., Winberg J. ( 1989 ). Elimination of vaginal colonization with Escherichia coli by administration of indigenous flora. . Infect Immun 57, 2447–2451.[PubMed]
    [Google Scholar]
  6. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  7. Kitahara M., Sakamoto M., Benno Y. ( 2010 ). Lactobacillus similis sp. nov., isolated from fermented cane molasses. . Int J Syst Evol Microbiol 60, 187–190. [CrossRef] [PubMed]
    [Google Scholar]
  8. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. & other authors ( 2007 ). clustal w and clustal_x version 2.0. . Bioinformatics 23, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  9. Lawson P. A., Falsen E., Ohlén M., Collins M. D. ( 2001a ). Aerococcus urinaehominis sp. nov., isolated from human urine. . Int J Syst Evol Microbiol 51, 683–686. [CrossRef] [PubMed]
    [Google Scholar]
  10. Lawson P. A., Falsen E., Truberg-Jensen K., Collins M. D. ( 2001b ). Aerococcus sanguicola sp. nov., isolated from a human clinical source. . Int J Syst Evol Microbiol 51, 475–479. [CrossRef] [PubMed]
    [Google Scholar]
  11. Otero C., Saavedra L., Silva de Ruiz C., Wilde O., Holgado A. R., Nader-Macías M. E. ( 2000 ). Vaginal bacterial microflora modifications during the growth of healthy cows. . Lett Appl Microbiol 31, 251–254. [CrossRef] [PubMed]
    [Google Scholar]
  12. Redondo-Lopez V., Cook R. L., Sobel J. D. ( 1990 ). Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. . Rev Infect Dis 12, 856–872. [CrossRef] [PubMed]
    [Google Scholar]
  13. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  14. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y. ( 2002 ). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.. Int J Syst Evol Microbiol 52, 841–849. [CrossRef] [PubMed]
    [Google Scholar]
  15. Sheldon I. M., Dobson H. ( 2004 ). Postpartum uterine health in cattle. . Anim Reprod Sci 82-83, 295–306. [CrossRef] [PubMed]
    [Google Scholar]
  16. Sheldon I. M., Noakes D. E., Rycroft A. N., Pfeiffer D. U., Dobson H. ( 2002 ). Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. . Reproduction 123, 837–845. [CrossRef] [PubMed]
    [Google Scholar]
  17. Sheldon I. M., Lewis G. S., LeBlanc S., Gilbert R. O. ( 2006 ). Defining postpartum uterine disease in cattle. . Theriogenology 65, 1516–1530. [CrossRef] [PubMed]
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  19. Tohno M., Kobayashi H., Nomura M., Kitahara M., Ohkuma M., Uegaki R., Cai Y. ( 2012a ). Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage. . Anim Sci J 83, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  20. Tohno M., Kobayashi H., Nomura M., Uegaki R., Cai Y. ( 2012b ). Identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage. . Anim Sci J 83, 318–330. [CrossRef] [PubMed]
    [Google Scholar]
  21. Tohno M., Kitahara M., Inoue H., Uegaki R., Irisawa T., Ohkuma M., Tajima K. ( 2013a ). Weissella oryzae sp. nov., isolated from fermented rice grains. . Int J Syst Evol Microbiol 63, 1417–1420. [CrossRef] [PubMed]
    [Google Scholar]
  22. Tohno M., Kitahara M., Irisawa T., Inoue H., Uegaki R., Ohkuma M., Tajima K. ( 2013b ). Lactobacillus oryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica). . Int J Syst Evol Microbiol 63, 2957–2962. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tohno M., Kitahara M., Irisawa T., Masuda T., Uegaki R., Ohkuma M., Tajima K. ( 2013c ). Description of Lactobacillus iwatensis sp. nov., isolated from orchardgrass (Dactylis glomerata L.) silage, and Lactobacillus backii sp. nov. . Int J Syst Evol Microbiol 63, 3854–3860. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tohno M., Kitahara M., Irisawa T., Masuda T., Uegaki R., Ohkuma M., Tajima K. ( 2013d ). Lactobacillus silagei sp. nov., isolated from orchardgrass silage. . Int J Syst Evol Microbiol 63, 4613–4618. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tohno M., Kitahara M., Uegaki R., Irisawa T., Ohkuma M., Tajima K. ( 2013e ). Lactobacillus hokkaidonensis sp. nov., isolated from subarctic timothy grass (Phleum pratense L.) silage. . Int J Syst Evol Microbiol 63, 2526–2531. [CrossRef] [PubMed]
    [Google Scholar]
  26. Vela A. I., García N., Latre M. V., Casamayor A., Sánchez-Porro C., Briones V., Ventosa A., Domínguez L., Fernández-Garayzábal J. F. ( 2007 ). Aerococcus suis sp. nov., isolated from clinical specimens from swine. . Int J Syst Evol Microbiol 57, 1291–1294. [CrossRef] [PubMed]
    [Google Scholar]
  27. Vintiñi E., Ocaña V., Elena Nader-Macías M. ( 2004 ). Effect of lactobacilli administration in the vaginal tract of mice: evaluation of side effects and local immune response by local administration of selected strains. . Methods Mol Biol 268, 401–410.[PubMed]
    [Google Scholar]
  28. Wang Y., Ametaj B. N., Ambrose D. J., Gänzle M. G. ( 2013 ). Characterisation of the bacterial microbiota of the vagina of dairy cows and isolation of pediocin-producing Pediococcus acidilactici . . BMC Microbiol 13, 19. [CrossRef] [PubMed]
    [Google Scholar]
  29. Williams R. E., Hirch A., Cowan S. T. ( 1953 ). Aerococcus, a new bacterial genus. . J Gen Microbiol 8, 475–480. [CrossRef] [PubMed]
    [Google Scholar]
  30. Williams E. J., Fischer D. P., Pfeiffer D. U., England G. C., Noakes D. E., Dobson H., Sheldon I. M. ( 2005 ). Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. . Theriogenology 63, 102–117. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.058081-0
Loading
Aerococcus vaginalis sp. nov., isolated from the vaginal mucosa of a beef cow, and emended descriptions of Aerococcus suis, Aerococcus viridans, Aerococcus urinaeequi, Aerococcus urinaehominis, Aerococcus urinae, Aerococcus christensenii and Aerococcus sanguinicola
Int J Syst Evol Microbiol 64, 1229 (2014); https://doi.org/10.1099/ijs.0.058081-0
/content/journal/ijsem/10.1099/ijs.0.058081-0
/content/journal/ijsem/10.1099/ijs.0.058081-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error