1887

International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue 4

sp. nov., isolated from the vaginal mucosa of a beef cow, and emended descriptions of , , , , , and Free

Abstract

A gram-stain-positive, facultatively anaerobic, non-spore-forming, catalase-negative, coccoid-shaped bacterial strain, designated BV2, was isolated from the vaginal mucosa of a beef cow in Japan. Phylogenetic analysis showed that the isolate shared high 16S rRNA gene sequence similarity (92.9 %) with 1821/02 and low similarity (<92.7 %) with any other recognized species of the genus . The DNA G+C content was 44.7 mol%, which is within the range observed among species of the genus (37.5–48.4 mol%). The major cellular fatty acid was Cω9, similar to other type strains of species of the genus . The results of genotypic, phenotypic and chemotaxonomic analyses as well as the low degree of DNA–DNA relatedness with all recognized members of the genus indicate that strain BV2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BV2 ( = JCM 19163 = DSM 27293). Emended descriptions of , , , , , and are also presented.

  • Published Online:
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.058081-0
2014-04-01
2020-01-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/4/1229.html?itemId=/content/journal/ijsem/10.1099/ijs.0.058081-0&mimeType=html&fmt=ahah

References

  1. Aguirre M., Collins M. D.. ( 1992;). Phylogenetic analysis of some Aerococcus-like organisms from urinary tract infections: description of Aerococcus urinae sp. nov.. J Gen Microbiol 138:, 401––405. [CrossRef][PubMed]
     [Google Scholar]
  2. Collins M. D., Jovita M. R., Hutson R. A., Ohlén M., Falsen E.. ( 1999;). Aerococcus christensenii sp. nov., from the human vagina. . Int J Syst Bacteriol 49:, 1125––1128. [CrossRef][PubMed]
     [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane-filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224––229. [CrossRef]
     [Google Scholar]
  4. Felis G. E., Torriani S., Dellaglio F.. ( 2005;). Reclassification of Pediococcus urinaeequi (ex Mees 1934) Garvie 1988 as Aerococcus urinaeequi comb. nov.. Int J Syst Evol Microbiol 55:, 1325––1327. [CrossRef][PubMed]
     [Google Scholar]
  5. Herthelius M., Gorbach S. L., Möllby R., Nord C. E., Pettersson L., Winberg J.. ( 1989;). Elimination of vaginal colonization with Escherichia coli by administration of indigenous flora. . Infect Immun 57:, 2447––2451.[PubMed]
     [Google Scholar]
  6. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111––120. [CrossRef][PubMed]
     [Google Scholar]
  7. Kitahara M., Sakamoto M., Benno Y.. ( 2010;). Lactobacillus similis sp. nov., isolated from fermented cane molasses. . Int J Syst Evol Microbiol 60:, 187––190. [CrossRef][PubMed]
     [Google Scholar]
  8. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A.. & other authors ( 2007;). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947––2948. [CrossRef][PubMed]
     [Google Scholar]
  9. Lawson P. A., Falsen E., Ohlén M., Collins M. D.. ( 2001a;). Aerococcus urinaehominis sp. nov., isolated from human urine. . Int J Syst Evol Microbiol 51:, 683––686. [CrossRef][PubMed]
     [Google Scholar]
  10. Lawson P. A., Falsen E., Truberg-Jensen K., Collins M. D.. ( 2001b;). Aerococcus sanguicola sp. nov., isolated from a human clinical source. . Int J Syst Evol Microbiol 51:, 475––479. [CrossRef][PubMed]
     [Google Scholar]
  11. Otero C., Saavedra L., Silva de Ruiz C., Wilde O., Holgado A. R., Nader-Macías M. E.. ( 2000;). Vaginal bacterial microflora modifications during the growth of healthy cows. . Lett Appl Microbiol 31:, 251––254. [CrossRef][PubMed]
     [Google Scholar]
  12. Redondo-Lopez V., Cook R. L., Sobel J. D.. ( 1990;). Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. . Rev Infect Dis 12:, 856––872. [CrossRef][PubMed]
     [Google Scholar]
  13. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406––425.[PubMed]
     [Google Scholar]
  14. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y.. ( 2002;). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.. Int J Syst Evol Microbiol 52:, 841––849. [CrossRef][PubMed]
     [Google Scholar]
  15. Sheldon I. M., Dobson H.. ( 2004;). Postpartum uterine health in cattle. . Anim Reprod Sci 82-83:, 295––306. [CrossRef][PubMed]
     [Google Scholar]
  16. Sheldon I. M., Noakes D. E., Rycroft A. N., Pfeiffer D. U., Dobson H.. ( 2002;). Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. . Reproduction 123:, 837––845. [CrossRef][PubMed]
     [Google Scholar]
  17. Sheldon I. M., Lewis G. S., LeBlanc S., Gilbert R. O.. ( 2006;). Defining postpartum uterine disease in cattle. . Theriogenology 65:, 1516––1530. [CrossRef][PubMed]
     [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731––2739. [CrossRef][PubMed]
     [Google Scholar]
  19. Tohno M., Kobayashi H., Nomura M., Kitahara M., Ohkuma M., Uegaki R., Cai Y.. ( 2012a;). Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage. . Anim Sci J 83:, 111––120. [CrossRef][PubMed]
     [Google Scholar]
  20. Tohno M., Kobayashi H., Nomura M., Uegaki R., Cai Y.. ( 2012b;). Identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage. . Anim Sci J 83:, 318––330. [CrossRef][PubMed]
     [Google Scholar]
  21. Tohno M., Kitahara M., Inoue H., Uegaki R., Irisawa T., Ohkuma M., Tajima K.. ( 2013a;). Weissella oryzae sp. nov., isolated from fermented rice grains. . Int J Syst Evol Microbiol 63:, 1417––1420. [CrossRef][PubMed]
     [Google Scholar]
  22. Tohno M., Kitahara M., Irisawa T., Inoue H., Uegaki R., Ohkuma M., Tajima K.. ( 2013b;). Lactobacillus oryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica). . Int J Syst Evol Microbiol 63:, 2957––2962. [CrossRef][PubMed]
     [Google Scholar]
  23. Tohno M., Kitahara M., Irisawa T., Masuda T., Uegaki R., Ohkuma M., Tajima K.. ( 2013c;). Description of Lactobacillus iwatensis sp. nov., isolated from orchardgrass (Dactylis glomerata L.) silage, and Lactobacillus backii sp. nov. . Int J Syst Evol Microbiol 63:, 3854––3860. [CrossRef][PubMed]
     [Google Scholar]
  24. Tohno M., Kitahara M., Irisawa T., Masuda T., Uegaki R., Ohkuma M., Tajima K.. ( 2013d;). Lactobacillus silagei sp. nov., isolated from orchardgrass silage. . Int J Syst Evol Microbiol 63:, 4613––4618. [CrossRef][PubMed]
     [Google Scholar]
  25. Tohno M., Kitahara M., Uegaki R., Irisawa T., Ohkuma M., Tajima K.. ( 2013e;). Lactobacillus hokkaidonensis sp. nov., isolated from subarctic timothy grass (Phleum pratense L.) silage. . Int J Syst Evol Microbiol 63:, 2526––2531. [CrossRef][PubMed]
     [Google Scholar]
  26. Vela A. I., García N., Latre M. V., Casamayor A., Sánchez-Porro C., Briones V., Ventosa A., Domínguez L., Fernández-Garayzábal J. F.. ( 2007;). Aerococcus suis sp. nov., isolated from clinical specimens from swine. . Int J Syst Evol Microbiol 57:, 1291––1294. [CrossRef][PubMed]
     [Google Scholar]
  27. Vintiñi E., Ocaña V., Elena Nader-Macías M.. ( 2004;). Effect of lactobacilli administration in the vaginal tract of mice: evaluation of side effects and local immune response by local administration of selected strains. . Methods Mol Biol 268:, 401––410.[PubMed]
     [Google Scholar]
  28. Wang Y., Ametaj B. N., Ambrose D. J., Gänzle M. G.. ( 2013;). Characterisation of the bacterial microbiota of the vagina of dairy cows and isolation of pediocin-producing Pediococcus acidilactici. . BMC Microbiol 13:, 19. [CrossRef][PubMed]
     [Google Scholar]
  29. Williams R. E., Hirch A., Cowan S. T.. ( 1953;). Aerococcus, a new bacterial genus. . J Gen Microbiol 8:, 475––480. [CrossRef][PubMed]
     [Google Scholar]
  30. Williams E. J., Fischer D. P., Pfeiffer D. U., England G. C., Noakes D. E., Dobson H., Sheldon I. M.. ( 2005;). Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. . Theriogenology 63:, 102––117. [CrossRef][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.058081-0
Loading
Aerococcus vaginalis sp. nov., isolated from the vaginal mucosa of a beef cow, and emended descriptions of Aerococcus suis, Aerococcus viridans, Aerococcus urinaeequi, Aerococcus urinaehominis, Aerococcus urinae, Aerococcus christensenii and Aerococcus sanguinicola
Int J Syst Evol Microbiol 64, 1229 (2014); https://doi.org/10.1099/ijs.0.058081-0
/content/journal/ijsem/10.1099/ijs.0.058081-0
/content/journal/ijsem/10.1099/ijs.0.058081-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error