1887

Abstract

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterial strain, THG-DR3, was isolated from soil of a pine grove in Jeju island, Republic of Korea. Strain THG-DR3 grew optimally at 20–25 °C, at pH 7.0–8.0 and in R2A broth without added NaCl. 16S rRNA gene sequence analysis indicated that strain THG-DR3 belongs to the genus . The closest relatives based on 16S rRNA gene sequence similarity were AR107 (98.0 %), NWER-II11 (97.7 %), G-1 (97.7 %) and DCY49 (97.4 %). The G+C content of the genomic DNA of strain THG-DR3 was 47.5 mol%. Levels of DNA–DNA relatedness between strain THG-DR3 and its closest phylogenetic neighbours were below 44.7 %. The presence of menaquinone 7 and of iso-C and summed feature 3 (comprising Cω7 and/or Cω6) as the major fatty acids supported the affiliation of strain THG-DR3 to the genus . The polar lipids of strain THG-DR3 consisted of phosphatidylethanolamine, three unidentified aminophospholipids, five unidentified aminolipids and three unidentified lipids. The results of physiological and biochemical tests allowed the differentiation of strain THG-DR3 from recognized species of the genus . Strain THG-DR3 ( = KACC 17172 = JCM 18824) is proposed as the type strain of a novel species of the genus , sp. nov. An emended description of the genus is also proposed.

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2014-05-01
2019-10-19
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