sp. nov., isolated from leaf mould Free

Abstract

A Gram-staining-negative, strictly aerobic, rod-shaped, pale-pink pigmented bacterial strain, designated TF8, was isolated from leaf mould in Cheonan, Republic of Korea. Its taxonomic position was determined through a polyphasic approach. Optimal growth occurred on R2A agar without NaCl supplementation, at 25–28 °C and at pH 6.0–7.0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TF8 belongs to the genus in the family . The sequence similarity between 16S rRNA genes of strain TF8 and the type strains of other species of the genus ranged from 92.1 to 94.7 %. The closest relatives of strain TF8 were BR-3 (94.7 %), R9-65 (94.5 %), BR-18 (94.5 %), WPCB133 (94.0 %) and Jip 10 (93.8 %). The major isoprenoid quinone was MK-7 and the major cellular fatty acids were iso-C (33.0 %), summed feature 3 (Cω7 and/or Cω6; 24.8 %) and summed feature 8 (Cω7 and/or Cω6; 13.0 %). The major polar lipids of TF8 were phosphatidylethanolamine and three unidentified aminophospholipids. The G+C content of the genomic DNA was 46.2 mol%. On the basis of the data presented here, strain TF8 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TF8 ( = KACC 17468 = JCM 19323).

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2014-07-01
2024-03-29
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