1887

Abstract

A Gram-staining-negative, strictly aerobic, rod-shaped, pale-pink pigmented bacterial strain, designated TF8, was isolated from leaf mould in Cheonan, Republic of Korea. Its taxonomic position was determined through a polyphasic approach. Optimal growth occurred on R2A agar without NaCl supplementation, at 25–28 °C and at pH 6.0–7.0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TF8 belongs to the genus in the family . The sequence similarity between 16S rRNA genes of strain TF8 and the type strains of other species of the genus ranged from 92.1 to 94.7 %. The closest relatives of strain TF8 were BR-3 (94.7 %), R9-65 (94.5 %), BR-18 (94.5 %), WPCB133 (94.0 %) and Jip 10 (93.8 %). The major isoprenoid quinone was MK-7 and the major cellular fatty acids were iso-C (33.0 %), summed feature 3 (Cω7 and/or Cω6; 24.8 %) and summed feature 8 (Cω7 and/or Cω6; 13.0 %). The major polar lipids of TF8 were phosphatidylethanolamine and three unidentified aminophospholipids. The G+C content of the genomic DNA was 46.2 mol%. On the basis of the data presented here, strain TF8 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TF8 ( = KACC 17468 = JCM 19323).

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2014-07-01
2021-10-18
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References

  1. An D.-S., Yin C.-R., Lee S.-T., Cho C.-H. ( 2009 ). Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. . Int J Syst Evol Microbiol 59, 11221125. [View Article] [PubMed]
    [Google Scholar]
  2. Anthony E. E. Jr ( 1931 ). A note on capsule staining. . Science 73, 319320. [View Article] [PubMed]
    [Google Scholar]
  3. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N. ( 2010 ). Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter . . Int J Syst Evol Microbiol 60, 134139. [View Article] [PubMed]
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  5. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 18611868.[PubMed]
    [Google Scholar]
  6. Collins M. D. ( 1985 ). Analysis of isoprenoid quinones. . Methods Microbiol 18, 239366.
    [Google Scholar]
  7. Cui C. H., Choi T. E., Yu H., Jin F., Lee S. T., Kim S. C., Im W. T. ( 2011 ). Mucilaginibacter composti sp. nov., with ginsenoside converting activity, isolated from compost. . J Microbiol 49, 393398. [View Article] [PubMed]
    [Google Scholar]
  8. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  9. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  10. Gomori G. ( 1955 ). Preparation of buffers for use in enzyme studies. . Methods Enzymol 1, 138146. [View Article]
    [Google Scholar]
  11. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  12. Han S. I., Lee H. J., Lee H. R., Kim K. K., Whang K. S. ( 2012 ). Mucilaginibacter polysacchareus sp. nov., an exopolysaccharide-producing bacterial species isolated from the rhizoplane of the herb Angelica sinensis . . Int J Syst Evol Microbiol 62, 632637. [View Article] [PubMed]
    [Google Scholar]
  13. Jeon Y., Lee S.-S., Chung B. S., Kim J. M., Bae J.-W., Park S. K., Jeon C. O. ( 2009 ). Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. . Int J Syst Evol Microbiol 59, 14511454. [View Article] [PubMed]
    [Google Scholar]
  14. Jiang F., Dai J., Wang Y., Xue X., Xu M., Guo Y., Li W., Fang C., Peng F. ( 2012 ). Mucilaginibacter soli sp. nov., isolated from Arctic tundra soil. . Int J Syst Evol Microbiol 62, 16301635. [View Article] [PubMed]
    [Google Scholar]
  15. Joung Y., Joh K. ( 2011 ). Mucilaginibacter myungsuensis sp. nov., isolated from a mesotrophic artificial lake. . Int J Syst Evol Microbiol 61, 15061510. [View Article] [PubMed]
    [Google Scholar]
  16. Kang S.-J., Jung Y.-T., Oh K.-H., Oh T.-K., Yoon J.-H. ( 2011 ). Mucilaginibacter boryungensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 61, 15491553. [View Article] [PubMed]
    [Google Scholar]
  17. Khan H., Chung E. J., Kang D. Y., Jeon C. O., Chung Y. R. ( 2013 ). Mucilaginibacter jinjuensis sp. nov., with xylan-degrading activity. . Int J Syst Evol Microbiol 63, 12671272. [View Article] [PubMed]
    [Google Scholar]
  18. Kim B.-C., Lee K. H., Kim M. N., Lee J., Shin K.-S. ( 2011 ). Mucilaginibacter dorajii sp. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 133. . Int J Syst Evol Microbiol 61, 13. [View Article]
    [Google Scholar]
  19. Kim B. C., Poo H., Lee K. H., Kim M. N., Kwon O. Y., Shin K. S. ( 2012a ). Mucilaginibacter angelicae sp. nov., isolated from the rhizosphere of Angelica polymorpha Maxim. . Int J Syst Evol Microbiol 62, 5560. [View Article] [PubMed]
    [Google Scholar]
  20. Kim J. H., Kang S. J., Jung Y. T., Oh T. K., Yoon J. H. ( 2012b ). Mucilaginibacter lutimaris sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 62, 515519. [View Article] [PubMed]
    [Google Scholar]
  21. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012c ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  22. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  23. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  24. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  25. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C. ( 2009 ). Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. . Int J Syst Evol Microbiol 59, 14471450. [View Article] [PubMed]
    [Google Scholar]
  26. Madhaiyan M., Poonguzhali S., Lee J.-S., Senthilkumar M., Lee K. C., Sundaram S. ( 2010 ). Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. . Int J Syst Evol Microbiol 60, 24512457. [View Article] [PubMed]
    [Google Scholar]
  27. Männistö M. K., Tiirola M., McConnell J., Häggblom M. M. ( 2010 ). Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. . Int J Syst Evol Microbiol 60, 28492856. [View Article] [PubMed]
    [Google Scholar]
  28. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  29. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  30. Montero-Calasanz M. C., Göker M., Rohde M., Spröer C., Schumann P., Busse H.-J., Schmid M., Tindall B. J., Klenk H.-P., Camacho M. ( 2013 ). Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium . . Int J Syst Evol Microbiol 63, 43864395. [View Article] [PubMed]
    [Google Scholar]
  31. Moore D. D., Dowhan D. ( 1995 ). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 211. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. . New York:: Wiley;.
    [Google Scholar]
  32. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N. ( 2007 ). Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. . Int J Syst Evol Microbiol 57, 23492354. [View Article] [PubMed]
    [Google Scholar]
  33. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  34. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
    [Google Scholar]
  35. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  36. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  37. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M. ( 2008 ). Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. . Int J Syst Evol Microbiol 58, 20462050. [View Article] [PubMed]
    [Google Scholar]
  38. Yoon J. H., Lee S. T., Park Y. H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187194. [View Article] [PubMed]
    [Google Scholar]
  39. Yoon J. H., Kang S. J., Park S., Oh T. K. ( 2012 ). Mucilaginibacter litoreus sp. nov., isolated from marine sand. . Int J Syst Evol Microbiol 62, 28222827. [View Article] [PubMed]
    [Google Scholar]
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