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Abstract

A taxonomic study using a polyphasic approach was performed on a Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated strain PB156, isolated from grass soil. Comparative 16S rRNA gene sequence studies showed that the isolate was clearly affiliated with the phylum , and most closely related to PB17, VUG-A42aa and VUG-A130, showing 96.4, 96.2 and 95.9 % 16S rRNA gene sequence similarity, respectively, while all other species of the genus shared only 89.3–95.2 % similarity. The main polyamine present was -homospermidine. The predominant menaquinone was MK-7. The major fatty acids were C iso, summed feature 3 (Cω6 and/or Cω7/), Cω5 and C anteiso. The G+C content of the genomic DNA of strain PB156 was 61.7 mol%. The combined genotypic and phenotypic data supported the conclusion that strain PB156 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PB156 ( = KCTC 32477 = JCM 19433).

Funding
This study was supported by the:
  • , Advanced Biomass R&D Centre
  • , Global Frontier Program
  • , Korean Ministry of Education, Science & Technology
  • , CAER (Centre for Aquatic Ecosystem Restoration) in the Eco-STAR project from the Korean Ministry of Environment
  • , Open Research Program of KIST (Korea Research of Science and Technology)
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2014-03-01
2020-08-03
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