1887

Abstract

A novel Gram-stain–positive, filamentous bacterial strain (A-T 7458) was isolated from soil collected from hill evergreen forest in Thailand. The strain developed cylindrical sporangia containing a longitudinal pair of motile spores, on short ramifications of aerial mycelia. From a taxonomic study using a polyphasic approach, strain A-T 7458 had typical characteristics of members of the genus . 16S rRNA gene sequence analysis indicated that the isolate was closely related to A-T 4600 (98.5 %), JCM 3166 (97.6 %) and NBRC 13918 (97.6 %). The DNA–DNA relatedness values, which differentiated the novel strain from the closest species, were significantly below 70 %. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinone was MK-9(H). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C and C, and saturated C and C. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the novel isolate is proposed to represent a novel species of the genus , to be named sp. nov. The type strain is A-T 7458 ( = BCC 48396 = NBRC 109077).

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2014-04-01
2019-10-22
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