1887

Abstract

The taxonomic status of a carbendazim-degrading strain, mbc-2, isolated from soil under the long-term application of carbendazim in China was determined by means of a polyphasic study. The cells were Gram-stain-positive, motile and rod-shaped. Strain mbc-2 grew optimally at pH 7.0, 30–35 °C and in the presence of 1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain mbc-2 fell within the genus , forming a coherent cluster with the type strain of , with which it exhibited 16S rRNA gene sequence similarity values of 97.9 %. The chemotaxonomic properties of strain mbc-2 were consistent with those of the genus : the cell-wall peptidoglycan type was based on -2,6-diaminopimelic acid, the predominant menaquinone was MK-8 (H) and the major fatty acid was iso-C. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, unknown phospholipids and an unknown aminolipid. The DNA G+C content was 72 mol%. Strain mbc-2 exhibited DNA–DNA relatedness values of 12.5±1.5 %, 23.7±2.7 % and 26.3±3.2 % with respect to DS-30, GW-9 and OS4. On the basis of the data obtained, strain mbc-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is mbc-2 ( = KACC 17152 = CCTCC AB 2012934).

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2014-06-01
2019-10-19
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