An endophytic actinomycete, strain R10-82T, isolated from surface-sterilized roots of rice (Oryza sativa L.) was studied using a polyphasic approach. Strain R10-82T produced branching substrate mycelia and developed spherical spore vesicles on aerial hyphae containing non-motile spores. The major cellular fatty acids were iso-C16 : 0, iso-C14 : 0 and 10-methyl C17 : 0. The predominant menaquinones were MK-9, MK-9(H2), MK-9(H4) and MK-9(H6). Rhamnose, ribose, madurose, mannose and glucose were detected in whole-cell hydrolysates. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannosides, hydroxylphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. These morphological and chemotaxonomic data were similar to those of the genus Sphaerisporangium. Analysis of the 16S rRNA gene sequence revealed that strain R10-82T was related most closely to Sphaerisporangium cinnabarinum JCM 3291T (98.3 % similarity). The DNA G+C content of strain R10-82T was 74 mol%. DNA–DNA relatedness data in combination with differences in the biochemical and physiological properties suggested that strain R10-82T should be classified as representing a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium rufum is proposed. The type strain is R10-82T ( = BCC 51287T = NBRC 109079T). An emended description of the genus Sphaerisporangium is also provided.
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Funding
This study was supported by the:
Higher Education Research Promotion and National Research University Project of Thailand
Office of the Higher Education Commission
Kitasato Institute for Life Sciences, Kitasato University, Japan
AraI.,
KudoT.(2007).Sphaerosporangium gen. nov., a new member of the family Streptosporangiaceae, with descriptions of three new species as Sphaerosporangium melleum sp. nov., Sphaerosporangium rubeum sp. nov. and Sphaerosporangium cinnabarinum sp. nov., and transfer of Streptosporangium viridialbum Nonomura and Ohara 1960 to Sphaerosporangium viridialbum comb. nov.. Actinomycetologica21, 11–21. [View Article]
BeckerB.,
LechevalierM. P.,
LechevalierH. A.(1965). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. . Appl Microbiol13, 236–243.[PubMed]
CaoY. R.,
JiangY.,
XuL. H.,
JiangC. L.(2009).Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China. . Int J Syst Evol Microbiol59, 1679–1684. [View Article][PubMed]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol100, 221–230. [View Article][PubMed]
EzakiT.,
HashimotoY.,
YabuuchiE.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol39, 224–229. [View Article]
FelsensteinJ.(1993).phylip (phylogeny inference package) version 3.5.1. . Distributed by the author. Department of Genome Sciences, University of Washington;, Seattle, USA:.
GordonR. E.,
BarnettD. A.,
HanderhanJ. E.,
PangC. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the Nocardia strain. . Int J Syst Bacteriol24, 54–63. [View Article]
HasegawaT.,
TakizawaM.,
TanidaS.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol29, 319–322. [View Article]
JukesT. H.,
CantorC. R.(1969). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by
MunroH. N.
. New York:: Academic Press;. [View Article]
KataokaM.,
UedaK.,
KudoT.,
SekiT.,
YoshidaT.(1997). Application of the variable region in 16S rDNA to create an index for rapid species identification in the genus Streptomyces
. . FEMS Microbiol Lett151, 249–255. [View Article][PubMed]
KroppenstedtR. M.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by
GoodfellowM.,
MinnikinD. E.
. London:: Academic Press;.
LechevalierM. P.,
LechevalierH.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol20, 435–443. [View Article]
MinnikinD. E.,
PatelP. V.,
AlshamaonyL.,
GoodfellowM.(1977). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol27, 104–117. [View Article]
TamaokaJ.,
KomagataK.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett25, 125–128. [View Article]
UchidaK.,
AidaK.(1977). Acyl type of bacterial cell wall: its simple identification by a colorimetric method. . J Gen Appl Microbiol23, 249–260. [View Article]
UchidaK.,
AidaK. O.(1984). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. . J Gen Appl Microbiol30, 131–134. [View Article]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.& other authors (1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]