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Volume 64, Issue Pt_5

sp. nov., isolated from soil Free

Abstract

Two Gram-stain-positive, rod-shaped and endospore-forming bacteria, designated WM-1 and WM-4, were isolated from a paddy soil and a forest soil, respectively, in South China. Comparative 16S rRNA gene sequence analyses showed that both strains were members of the genus and most closely related to LMG 23627 with pairwise sequence similarity of 96.0 %. The isolates contained menaquinone-7 (MK-7) as the respiratory quinone and anteiso-C, anteiso-C and iso-C as the major fatty acids (>10 %). Polar lipids consisted of a predominance of diphosphatidylglycerol and moderate to minor amounts of phosphatidylglycerol and phosphatidylinositol. The cell-wall peptidoglycan contained -diaminopimelic acid. The DNA GC content was 38.6–39.2 mol%. The 16S rRNA gene sequence of strain WM-1 displayed 99.7 % similarity to that of strain WM-4, and DNA–DNA hybridization between the two strains showed a relatedness value of 91 %. Based on the results of this polyphasic study, strains WM-1 and WM-4 represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is WM-1 ( = KCTC 33119 = CGMCC 1.12406).

  • Published Online:
Funding
This study was supported by the:
  • Team Project of Guangdong Natural Science Foundation (Award S2012030006114 and S2011030002882)
  • Science and Technology Planning Project of Guangdong Province, China (Award 2012B010500035 and 2012B030800008)
  • Guangzhou Science and Technology Development Foundation, China (Award 2011J2200039)
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2014-05-01
2024-04-25
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Oceanobacillusluteolus sp. nov., isolated from soil
Int J Syst Evol Microbiol 64, 1495 (2014); https://doi.org/10.1099/ijs.0.057869-0
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