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Abstract

Bacterial strain M47C3B was isolated from the endophytic microbial community of a tree branch from a mixed grove of pines. Phylogenetic analysis of 16S rRNA gene sequences showed that this organism represented one distinct branch within the family , most closely related to the genus . Strain M47C3B formed a distinct lineage, closely related to KACC 14556, with which it shared 97.2 % 16S rRNA gene sequence similarity. The other members of the genus included in the same clade were ATCC BAA-1855 sharing 97.0 % similarity and TR6-03 that had a lower similarity (95.7 %). The novel strain was Gram-staining-negative, formed rod-shaped cells, grew optimally at 26 °C and at pH 7, and was able to grow with up to 0.3 % (w/v) NaCl. The respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids of the strain were summed feature 3 (Cω7/iso-C 2-OH), iso-C and iso-C 3-OH, representing 73.5 % of the total fatty acids. The major components of the polar lipid profile of strain M47C3B consisted of phosphatidylethanolamine, three unidentified aminophospholipids, one unidentified aminolipid and three unidentified polar lipids. The G+C content of the DNA was 40.6 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics we propose the name sp. nov. for the novel species represented by strain M47C3B ( = CIP 110632 = LMG 28160).

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2014-07-01
2020-01-24
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