1887

Abstract

Bacterial strain M47C3B was isolated from the endophytic microbial community of a tree branch from a mixed grove of pines. Phylogenetic analysis of 16S rRNA gene sequences showed that this organism represented one distinct branch within the family , most closely related to the genus . Strain M47C3B formed a distinct lineage, closely related to KACC 14556, with which it shared 97.2 % 16S rRNA gene sequence similarity. The other members of the genus included in the same clade were ATCC BAA-1855 sharing 97.0 % similarity and TR6-03 that had a lower similarity (95.7 %). The novel strain was Gram-staining-negative, formed rod-shaped cells, grew optimally at 26 °C and at pH 7, and was able to grow with up to 0.3 % (w/v) NaCl. The respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids of the strain were summed feature 3 (Cω7/iso-C 2-OH), iso-C and iso-C 3-OH, representing 73.5 % of the total fatty acids. The major components of the polar lipid profile of strain M47C3B consisted of phosphatidylethanolamine, three unidentified aminophospholipids, one unidentified aminolipid and three unidentified polar lipids. The G+C content of the DNA was 40.6 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics we propose the name sp. nov. for the novel species represented by strain M47C3B ( = CIP 110632 = LMG 28160).

Funding
This study was supported by the:
  • , Program of National Action for Pine Wood Nematode control: ‘The disease of Pine Wood Nematode’
  • , Instituto de Financiamento da Agricultura e Pescas (IFAP)
  • , Autoridade Florestal Nacional (AFN)
  • , Fundação para a Ciência e Tecnologia (FCT), Portugal , (Award PTDC/AGR-CFL/115373/2009)
  • , FCT, Portugal , (Award DRH36/11/448)
  • , FCT, Portugal , (Award SFRH/BD/61311/2009)
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2014-07-01
2020-11-28
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References

  1. An D.-S., Yin C.-R., Lee S.-T., Cho C.-H. ( 2009 ). Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. . Int J Syst Evol Microbiol 59, 11221125. [CrossRef] [PubMed]
    [Google Scholar]
  2. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N. ( 2010 ). Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter . . Int J Syst Evol Microbiol 60, 134139. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cui C.-H., Choi T.-E., Yu H., Jin F., Lee S.-T., Kim S.-C., Im W.-T. ( 2011 ). Mucilaginibacter composti sp. nov., with ginsenoside converting activity, isolated from compost. . J Microbiol 49, 393398. [CrossRef] [PubMed]
    [Google Scholar]
  4. da Costa M. S., Nobre M. F., Wait R. ( 2006 ). Analysis of lipids from extremophilic bacteria. . Methods Microbiol 35, 127159. [CrossRef]
    [Google Scholar]
  5. da Costa M. S., Albuquerque L., Nobre M. F., Wait R. ( 2011a ). The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. . Methods Microbiol 38, 197206. [CrossRef]
    [Google Scholar]
  6. da Costa M. S., Albuquerque L., Nobre M. F., Wait R. ( 2011b ). The identification of fatty acids in bacteria. . Methods Microbiol 38, 183196. [CrossRef]
    [Google Scholar]
  7. Fautz E., Reichenbach H. ( 1980 ). A simple test for flexirubin-type pigments. . FEMS Microbiol Lett 8, 8791. [CrossRef]
    [Google Scholar]
  8. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  9. Freeman D. J., Falkiner F. R., Keane C. T. ( 1989 ). New method for detecting slime production by coagulase negative staphylococci. . J Clin Pathol 42, 872874. [CrossRef] [PubMed]
    [Google Scholar]
  10. Han S.-I., Lee H.-J., Lee H.-R., Kim K.-K., Whang K.-S. ( 2012 ). Mucilaginibacter polysacchareus sp. nov., an exopolysaccharide-producing bacterial species isolated from the rhizoplane of the herb Angelica sinensis . . Int J Syst Evol Microbiol 62, 632637. [CrossRef] [PubMed]
    [Google Scholar]
  11. Jeon Y., Lee S.-S., Chung B. S., Kim J. M., Bae J.-W., Park S. K., Jeon C. O. ( 2009 ). Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. . Int J Syst Evol Microbiol 59, 14511454. [CrossRef] [PubMed]
    [Google Scholar]
  12. Jiang F., Dai J., Wang Y., Xue X., Xu M., Guo Y., Li W., Fang C., Peng F. ( 2012 ). Mucilaginibacter soli sp. nov., isolated from Arctic tundra soil. . Int J Syst Evol Microbiol 62, 16301635. [CrossRef] [PubMed]
    [Google Scholar]
  13. Joung Y., Joh K. ( 2011 ). Mucilaginibacter myungsuensis sp. nov., isolated from a mesotrophic artificial lake. . Int J Syst Evol Microbiol 61, 15061510. [CrossRef] [PubMed]
    [Google Scholar]
  14. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  15. Kang S.-J., Jung Y.-T., Oh K.-H., Oh T.-K., Yoon J.-H. ( 2011 ). Mucilaginibacter boryungensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 61, 15491553. [CrossRef] [PubMed]
    [Google Scholar]
  16. Khan H., Chung E. J., Kang D. Y., Jeon C. O., Chung Y. R. ( 2013 ). Mucilaginibacter jinjuensis sp. nov., with xylan-degrading activity. . Int J Syst Evol Microbiol 63, 12671272. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kim B.-C., Lee K. H., Kim M. N., Lee J., Shin K.-S. ( 2010 ). Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum . . FEMS Microbiol Lett 309, 130135.[PubMed]
    [Google Scholar]
  18. Kim B.-C., Poo H., Lee K. H., Kim M. N., Kwon O.-Y., Shin K.-S. ( 2012a ). Mucilaginibacter angelicae sp. nov., isolated from the rhizosphere of Angelica polymorpha Maxim. . Int J Syst Evol Microbiol 62, 5560. [CrossRef] [PubMed]
    [Google Scholar]
  19. Kim J.-H., Kang S.-J., Jung Y.-T., Oh T.-K., Yoon J.-H. ( 2012b ). Mucilaginibacter lutimaris sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 62, 515519. [CrossRef] [PubMed]
    [Google Scholar]
  20. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012c ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  21. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [CrossRef] [PubMed]
    [Google Scholar]
  22. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C. ( 2009 ). Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. . Int J Syst Evol Microbiol 59, 14471450. [CrossRef] [PubMed]
    [Google Scholar]
  23. Madhaiyan M., Poonguzhali S., Lee J.-S., Senthilkumar M., Lee K. C., Sundaram S. ( 2010 ). Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. . Int J Syst Evol Microbiol 60, 24512457. [CrossRef] [PubMed]
    [Google Scholar]
  24. Männistö M. K., Tiirola M., McConnell J., Häggblom M. M. ( 2010 ). Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. . Int J Syst Evol Microbiol 60, 28492856. [CrossRef] [PubMed]
    [Google Scholar]
  25. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  26. Morais P. V., Francisco R., Branco R., Chung A. P., da Costa M. S. ( 2004 ). Leucobacter chromiireducens sp. nov, and Leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment. . Syst Appl Microbiol 27, 646652. [CrossRef]
    [Google Scholar]
  27. Olsen G. J., Matsuda H., Hagstrom R., Overbeek R. ( 1994 ). fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. . Comput Appl Biosci 10, 4148.[PubMed]
    [Google Scholar]
  28. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N. ( 2007 ). Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. . Int J Syst Evol Microbiol 57, 23492354. [CrossRef] [PubMed]
    [Google Scholar]
  29. Pruesse E., Peplies J., Glöckner F. O. ( 2012 ). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28, 18231829. [CrossRef] [PubMed]
    [Google Scholar]
  30. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  31. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Microbiology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  32. Tindall B. J., Sikorski J., Simbert A. R., Krieg R. N. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330393. Edited by Reddy C. A., Beveridge J. T., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. . Washington:, DC:: American Society for Microbiology;.
    [Google Scholar]
  33. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M. ( 2008 ). Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. . Int J Syst Evol Microbiol 58, 20462050. [CrossRef] [PubMed]
    [Google Scholar]
  34. Yoon J.-H., Kang S.-J., Park S., Oh T.-K. ( 2012 ). Mucilaginibacter litoreus sp. nov., isolated from marine sand. . Int J Syst Evol Microbiol 62, 28222827. [CrossRef] [PubMed]
    [Google Scholar]
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