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Abstract

A bacterial strain designated CMJ-9 was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-9 were strictly aerobic, Gram-negative, motile by a single polar flagellum, poly-β-hydroxybutyrate-containing and formed light-yellow colonies. Growth occurred at 10–37 °C (optimum, 20–30 °C), with 0–0.8 % NaCl (optimum, 0–0.1 %) and at pH 6.0–9.0 (optimum, pH 6.0–7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-9 belonged to the genus , and its closest neighbour was SHS5-24, with 96.7 % sequence similarity. The predominant cellular fatty acids were summed feature 3 (Cω7 and/or Cω6) and C. The major cellular hydroxy fatty acid was C 3-OH. The polar lipid profile consisted of the predominant lipids phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 47.7 mol%. On the basis of the phylogenetic and phenotypic data, strain CMJ-9 should be classified within a novel species, for which the name sp. nov. is proposed. The type strain is CMJ-9 ( = BCRC 80406 = LMG 26891 = KCTC 23916).

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2014-03-01
2024-12-12
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