1887

Abstract

A Gram-reaction-negative, aerobic, non-spore-forming, non-motile and rod-shaped or ovoid bacterial strain, designated SSK6-1, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK6-1 grew optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSK6-1 clustered with TF-218 and LMG 24369, exhibiting sequence similarities of 98.6 and 98.3 %, respectively. The novel strain exhibited sequence similarities of 94.6–97.8 % to the type strains of other recognized species of the genera and . Strain SSK6-1 contained Q-10 as the predominant ubiquinone and Cω7 and 11-methyl Cω7 as the major fatty acids. The major polar lipids of strain SSK6-1 were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain SSK6-1 was 64.6 mol% and its DNA–DNA relatedness values with TF-218 and were 21 and 25 %, respectively. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain SSK6-1 is separate from recognized species. On the basis of the data presented, strain SSK6-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SSK6-1 ( = KCTC 32538 = CECT 8399).

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2014-04-01
2019-09-16
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References

  1. Barrow G. I., Feltham R. K. A.. (editors) ( 1993;). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Baumann P., Baumann L.. ( 1981;). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G... Berlin:: Springer;.
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L.. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef][PubMed]
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y.. ( 1957;). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. . J Cell Physiol 49:, 25–68. [CrossRef][PubMed]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  6. Gaboyer F., Tindall B. J., Ciobanu M.-C., Duthoit F., Le Romancer M., Alain K.. ( 2013;). Phaeobacter leonis sp. nov., an alphaproteobacterium from Mediterranean Sea sediments. . Int J Syst Evol Microbiol 63:, 3301–3306. [CrossRef][PubMed]
    [Google Scholar]
  7. Komagata K., Suzuki K.-I.. ( 1987;). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  8. Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  9. Martens T., Heidorn T., Pukall R., Simon M., Tindall B. J., Brinkhoff T.. ( 2006;). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. . Int J Syst Evol Microbiol 56:, 1293–1304. [CrossRef][PubMed]
    [Google Scholar]
  10. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  11. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  12. Ruiz-Ponte C., Cilia V., Lambert C., Nicolas J. L.. ( 1998;). Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus. . Int J Syst Bacteriol 48:, 537–542. [CrossRef][PubMed]
    [Google Scholar]
  13. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  14. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  15. Staley J. T.. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95:, 1921–1942.[PubMed]
    [Google Scholar]
  16. Sun F., Wang B., Liu X., Lai Q., Du Y., Li G., Luo J., Shao Z.. ( 2010;). Leisingera nanhaiensis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 60:, 275–280. [CrossRef][PubMed]
    [Google Scholar]
  17. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  18. Vandecandelaere I., Segaert E., Mollica A., Faimali M., Vandamme P.. ( 2009;). Phaeobacter caeruleus sp. nov., a blue-coloured, colony-forming bacterium isolated from a marine electroactive biofilm. . Int J Syst Evol Microbiol 59:, 1209–1214. [CrossRef][PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  20. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H.. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  21. Yoon J.-H., Lee S. T., Park Y.-H.. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef][PubMed]
    [Google Scholar]
  22. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H.. ( 2003;). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53:, 53–57. [CrossRef][PubMed]
    [Google Scholar]
  23. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh T.-K.. ( 2007;). Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 57:, 856–861. [CrossRef][PubMed]
    [Google Scholar]
  24. Zhang D.-C., Li H.-R., Xin Y.-H., Liu H.-C., Chi Z.-M., Zhou P.-J., Yu Y.. ( 2008;). Phaeobacter arcticus sp. nov., a psychrophilic bacterium isolated from the Arctic. . Int J Syst Evol Microbiol 58:, 1384–1387. [CrossRef][PubMed]
    [Google Scholar]
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