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Abstract

A novel halophilic, filamentous actinomycete, designated strain AFM 10251, was isolated from a sediment sample collected from the Dead Sea, Israel. The isolate grew with 10–35 % multi-salts, and did not grow without NaCl or MgCl. The isolate formed a white aerial mycelium, and long chains of arthrospores with more than 10 spores per chain. The spores were spherical or oval with warty surfaces, and sterile mycelium was present between individual spores. The isolate contained -diaminopimelic acid and a small proportion of -diaminopimelic acid as cell-wall diamino acids, and galactose and arabinose as whole-cell sugars. The major menaquinone was MK-9(H). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and three unknown phospholipids. Major fatty acids were iso-C, iso-C, iso-C and anteiso-C. The DNA G+C content of strain AFM 10251 was 66.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AFM 10251 and the genus formed a distinct lineage. Analysis of the secondary structures of variable areas of the 16S rRNA gene showed that strain AFM 10251 was different from all recognized species of the genus and members of the family . Analysis of the signature nucleotides of the 16S rRNA gene showed that strain AFM 10251 and formed a single group, but with base pair differences at positions 127 : 234 and 183 : 194. On the basis of analysis of chemical and molecular characteristics, strain AFM 10251 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is AFM 10251 ( = DSM 45976 = CGMCC 4.7114).

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2014-08-01
2020-11-30
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