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Volume 64, Issue Pt_5

gen. nov., sp. nov., isolated from mushroom residue compost Free

Abstract

A novel thermophilic actinomycete, designated AG2-7, was isolated from mushroom residue compost in Guangxi University, Nanning, China. The strain grew optimally at 45–60 °C, at pH 7.0 and with 0–3.0 % (w/v) NaCl. Vegetative mycelia were branched and whitish to pale yellow without fragmentation. Aerial mycelium was abundant, whitish and differentiated into long chains of spores, with a membranous structure or tunica partially covering the surface of aerial hyphae. The non-motile spores were oval in shape with a ridged surface. Strain AG-27 contained -diaminopimelic acid as the diagnostic diamino acid, and the whole-cell sugars were galactose and ribose. Major fatty acids were iso-C (27.51 %), iso-C (10.47 %) and anteiso-C (12.01 %). MK-9(H) was the predominant menaquinone. The polar phospholipids were diphosphatidylglycerol, ninhydrin-positive glycophospholipid, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylethanolamine, phosphatidylmethylethanolamine, an unknown phospholipid and unknown glucosamine-containing phospholipids. The G+C content of the genomic DNA was 63.6 mol%. 16S rRNA gene sequence analysis showed that the organism belonged to the family , suborder and showed more than 5 % divergence from other members of the family. Based on the phenotypic and phylogenetic data, strain AG2-7 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is AG2-7 ( = ATCC BAA-2499 = CGMCC 4.7099).

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Funding
This study was supported by the:
  • Guangxi Natural Science Foundation Key Programs (Award 2010GXNSFD013027)
  • National Natural Science Foundation of China (Award 30860006)
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 3389–3402. [View Article] [PubMed]
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 2259–2261. [View Article] [PubMed]
    [Google Scholar]
  3. Collins M. D., Howarth O. W., Grund E., Kroppenstedt R. M. ( 1987 ). Isolation and structural determination of new members of the vitamin K2 series in Nocardia brasiliensis . . FEMS Microbiol Lett 41, 35–39. [View Article]
    [Google Scholar]
  4. Embley M. T., Smida J., Stackebrandt E. ( 1988 ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and description of Pseudonocardiaceae fam. nov.. Syst Appl Microbiol 11, 44–52. [View Article]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  7. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  8. Gause G. F., Preobrazhenskaya T. P., Sveshnikova M. A., Terekhova L. P., Maximova T. S. ( 1983 ). A Guide for the Determination of Actinomycetes. Genera Streptomyces, Streptoverticillium and Chainia. Moscow:: Nauka;.
     [Google Scholar]
  9. Gochnauer M. B., Leppard G. G., Komaratat P., Kates M., Novitsky T., Kushner D. J. ( 1975 ). Isolation and characterization of Actinopolyspora halophila, gen. et sp. nov., an extremely halophilic actinomycete. . Can J Microbiol 21, 1500–1511. [View Article] [PubMed]
    [Google Scholar]
  10. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 54–63. [View Article]
    [Google Scholar]
  11. Hasegawa T., Lechevalier M. P., Lechevalier H. A. ( 1978 ). New genus of the Actinomycetales: Actinosynnema gen. nov.. Int J Syst Bacteriol 28, 304–310. [View Article]
    [Google Scholar]
  12. Hasegawa T., Takizawa M., Tanida S. ( 1983 ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29, 319–322. [View Article]
    [Google Scholar]
  13. Hayakawa M., Nonomura H. ( 1987 ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65, 501–509. [View Article]
    [Google Scholar]
  14. Henssen A. ( 1957 ). [ Morphology and system of thermophilic actinomycetes. ]. Arch Mikrobiol 26, 373–414 (in German). [View Article] [PubMed]
    [Google Scholar]
  15. Indananda C., Matsumoto A., Inahashi Y., Takahashi Y., Duangmal K., Thamchaipenet A. ( 2010 ). Actinophytocola oryzae gen. nov., sp. nov., isolated from the roots of Thai glutinous rice plants, a new member of the family Pseudonocardiaceae . . Int J Syst Evol Microbiol 60, 1141–1146. [View Article] [PubMed]
    [Google Scholar]
  16. Jiang Y., Wiese J., Tang S. K., Xu L. H., Imhoff J. F., Jiang C. L. ( 2008 ). Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae . . Int J Syst Evol Microbiol 58, 408–413. [View Article] [PubMed]
    [Google Scholar]
  17. Jones K. L. ( 1949 ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57, 141–145.[PubMed]
    [Google Scholar]
  18. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  19. Kim S. B., Goodfellow M. ( 1999 ). Reclassification of Amycolatopsis rugosa Lechevalier et al. 1986 as Prauserella rugosa gen. nov., comb. nov. . Int J Syst Bacteriol 49, 507–512. [View Article] [PubMed]
    [Google Scholar]
  20. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  21. Korn-Wendisch F., Rainey F., Kroppenstedt R. M., Kempf A., Majazza A., Kutzner H. J., Stackebrandt E. ( 1995 ). Thermocrispum gen. nov., a new genus of the order Actinomycetales, and description of Thermocrispum municipale sp. nov. and Thermocrispum agreste sp. nov.. Int J Syst Bacteriol 45, 67–77. [View Article] [PubMed]
    [Google Scholar]
  22. Kroppenstedt R. M. ( 1982 ). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5, 2359–2367. [View Article]
    [Google Scholar]
  23. Labeda D. P. ( 2001 ). Crossiella gen. nov., a new genus related to Streptoalloteichus . . Int J Syst Evol Microbiol 51, 1575–1579.[PubMed] [CrossRef]
    [Google Scholar]
  24. Labeda D. P., Kroppenstedt R. M. ( 2000 ). Phylogenetic analysis of Saccharothrix and related taxa: proposal for Actinosynnemataceae fam. nov.. Int J Syst Evol Microbiol 50, 331–336. [View Article] [PubMed]
    [Google Scholar]
  25. Labeda D. P., Kroppenstedt R. M. ( 2007 ). Proposal of Umezawaea gen. nov., a new genus of the Actinosynnemataceae related to Saccharothrix, and transfer of Saccharothrix tangerinus Kinoshita et al. 2000 as Umezawaea tangerina gen. nov., comb. nov.. Int J Syst Evol Microbiol 57, 2758–2761. [View Article] [PubMed]
    [Google Scholar]
  26. Labeda D. P., Kroppenstedt R. M. ( 2008 ). Proposal for the new genus Allokutzneria gen. nov. within the suborder Pseudonocardineae and transfer of Kibdelosporangium albatum Tomita et al. 1993 as Allokutzneria albata comb. nov.. Int J Syst Evol Microbiol 58, 1472–1475. [View Article] [PubMed]
    [Google Scholar]
  27. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A. ( 1984 ). Saccharothrix, a new genus of the Actinomycetales related to Nocardiopsis . . Int J Syst Bacteriol 34, 426–431. [View Article]
    [Google Scholar]
  28. Labeda D. P., Kroppenstedt R. M., Euzéby J. P., Tindall B. J. ( 2008 ). Proposal of Goodfellowiella gen. nov. to replace the illegitimate genus name Goodfellowia Labeda and Kroppenstedt 2006. . Int J Syst Evol Microbiol 58, 1047–1048. [View Article] [PubMed]
    [Google Scholar]
  29. Labeda D. P., Goodfellow M., Chun J., Zhi X.-Y., Li W.-J. ( 2011 ). Reassessment of the systematics of the suborder Pseudonocardineae: transfer of the genera within the family Actinosynnemataceae Labeda and Kroppenstedt 2000 emend. Zhi et al. 2009 into an emended family Pseudonocardiaceae Embley et al. 1989 emend. Zhi et al. 2009. . Int J Syst Evol Microbiol 61, 1259–1264. [View Article] [PubMed]
    [Google Scholar]
  30. Lacey J., Goodfellow M., Lacy J., Goodfellow M. ( 1975 ). A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et. sp. nov.. J Gen Microbiol 88, 75–85. [View Article] [PubMed]
    [Google Scholar]
  31. Lechevalier M. P., Lechevalier H. A. ( 1970 ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20, 435–443. [View Article]
    [Google Scholar]
  32. Lechevalier M. P., Lechevalier H. A. ( 1980 ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy ( Special Publication no. 6), pp. 227–291. Edited by Dietz A., Thayer J. . Arlington, VA:: Society for Industrial Microbiology;.
     [Google Scholar]
  33. Lechevalier M. P., Prauser H., Labeda D. P., Ruan J.-S. ( 1986 ). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. . Int J Syst Bacteriol 36, 29–37. [View Article]
    [Google Scholar]
  34. Lee S. D. ( 2012 ). Labedaea rhizosphaerae gen. nov., sp. nov., isolated from rhizosphere soil. . Int J Syst Evol Microbiol 62, 1772–1778. [View Article] [PubMed]
    [Google Scholar]
  35. Li W.-J., Xu P., Schumann P., Zhang Y.-Q., Pukall R., Xu L.-H., Stackebrandt E., Jiang C.-L. ( 2007 ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57, 1424–1428. [View Article] [PubMed]
    [Google Scholar]
  36. Lisdiyanti P., Otoguro M., Ratnakomala S., Lestari Y., Hastuti R. D., Triana E., Katsuhiko A., Widyastuti Y. ( 2010 ). Actinokineospora baliensis sp. nov., Actinokineospora cibodasensis sp. nov. and Actinokineospora cianjurensis sp. nov., isolated from soil and plant litter. . Int J Syst Evol Microbiol 60, 2331–2335. [View Article] [PubMed]
    [Google Scholar]
  37. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195–206. [View Article]
    [Google Scholar]
  38. Mao J., Wang J., Dai H.-Q., Zhang Z.-D., Tang Q.-Y., Ren B., Yang N., Goodfellow M., Zhang L. X., Liu Z.-H. ( 2011 ). Yuhushiella deserti gen. nov., sp. nov., a new member of the suborder Pseudonocardineae . . Int J Syst Evol Microbiol 61, 621–630. [View Article] [PubMed]
    [Google Scholar]
  39. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208–218. [View Article]
    [Google Scholar]
  40. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188, 221–233. [View Article]
    [Google Scholar]
  41. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  42. Mordarska H., Mordarski M., Goodfellow M. ( 1972 ). Chemotaxonomic characters and classification of some nocardioform bacteria. . J Gen Microbiol 71, 77–86. [View Article] [PubMed]
    [Google Scholar]
  43. Nonomura H., Ohara Y. ( 1971 ). Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes. . J Ferment Technol 49, 895–903.
     [Google Scholar]
  44. Otoguro M., Hayakawa M., Yamazaki T., Tamura T., Hatano K., Iimura Y. ( 2001 ). Numerical phenetic and phylogenetic analysis of Actinokineospora isolates, with a description of Actinokineospora auranticolor sp. nov. and Actinokineospora enzanensis sp. nov.. Actinomycetologica 15, 30–39. [View Article]
    [Google Scholar]
  45. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. ( 1996 ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46, 1088–1092. [View Article] [PubMed]
    [Google Scholar]
  46. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  47. Shearer M. C., Colman P. M., Ferrin R. M., Nisbet L. J., Nash C. H. III ( 1986 ). New genus of the Actinomycetales: Kibdelosporangium aridum gen. nov., sp. nov.. Int J Syst Bacteriol 36, 47–54. [View Article]
    [Google Scholar]
  48. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  49. Stackebrandt E., Kroppenstedt R. M., Jahnke K.-D., Kemmerling C., Gürtler H. ( 1994 ). Transfer of Streptosporangium viridogriseum (Okuda et al. 1966), Streptosporangium viridogriseum subsp. kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb. nov., Kutzneria kofuensis comb. nov., and Kutzneria albida comb. nov., respectively, and emendation of the genus Streptosporangium . . Int J Syst Bacteriol 44, 265–269. [View Article]
    [Google Scholar]
  50. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. ( 1997 ). Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47, 479–491. [View Article]
    [Google Scholar]
  51. Suriyachadkun C., Ngaemthao W., Chunhametha S., Tamura T., Sanglier J. J. ( 2013 ). Kutzneria buriramensis sp. nov., isolated from soil, and emended description of the genus Kutzneria . . Int J Syst Evol Microbiol 63, 47–52. [View Article] [PubMed]
    [Google Scholar]
  52. Tamura T., Hayakawa M., Nonomura H., Yokota A., Hatano K. ( 1995 ). Four new species of the genus Actinokineospora: Actinokineospora inagensis sp. nov., Actinokineospora globicatena sp. nov., Actinokineospora terrae sp. nov., and Actinokineospora diospyrosa sp. nov.. Int J Syst Bacteriol 45, 371–378. [View Article]
    [Google Scholar]
  53. Tamura T., Zhiheng L., Yamei Z., Hatano K. ( 2000 ). Actinoalloteichus cyanogriseus gen. nov., sp. nov.. Int J Syst Evol Microbiol 50, 1435–1440. [View Article] [PubMed]
    [Google Scholar]
  54. Tamura T., Ishida Y., Otoguro M., Hatano K., Suzuki K. ( 2008 ). Classification of ‘Streptomyces tenebrarius’ Higgins and Kastner as Streptoalloteichus tenebrarius nom. rev., comb. nov., and emended description of the genus Streptoalloteichus . . Int J Syst Evol Microbiol 58, 688–691. [View Article] [PubMed]
    [Google Scholar]
  55. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  56. Tang S. K., Wang Y., Zhang H., Lee J. C., Lou K., Kim C. J., Li W. J. ( 2010 ). Haloechinothrix alba gen. nov., sp. nov., a halophilic, filamentous actinomycete of the suborder Pseudonocardineae . . Int J Syst Evol Microbiol 60, 2154–2158. [View Article] [PubMed]
    [Google Scholar]
  57. Tang X., Zhou Y., Zhang J., Ming H., Nie G.-X., Yang L.-L., Tang S.-K., Li W.-J. ( 2012 ). Actinokineospora soli sp. nov., a thermotolerant actinomycete isolated from soil, and emended description of the genus Actinokineospora . . Int J Syst Evol Microbiol 62, 1845–1849. [View Article] [PubMed]
    [Google Scholar]
  58. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  59. Tian X. P., Zhi X. Y., Qiu Y. Q., Zhang Y. Q., Tang S. K., Xu L. H., Zhang S., Li W. J. ( 2009 ). Sciscionella marina gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea. . Int J Syst Evol Microbiol 59, 222–228. [View Article] [PubMed]
    [Google Scholar]
  60. Tomita K., Uenoyama Y., Numata E. I., Sasahira T., Hoshino Y., Fujisawa K. I., Tsukiura H., Kawaguchi H. ( 1978 ). Streptoalloteichus, a new genus of the family Actinoplanaceae . . J Antibiot (Tokyo) 31, 497–510. [View Article] [PubMed]
    [Google Scholar]
  61. Uchida K., Kudo T., Suzuki K. I., Nakase T. ( 1999 ). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. . J Gen Appl Microbiol 45, 49–56. [View Article] [PubMed]
    [Google Scholar]
  62. Waksman S. A. ( 1961 ). The Actinomycetes, vol. II. Classification, Identification and Description of Genera and Species. Baltimore:: Williams &Wilkins;.
     [Google Scholar]
  63. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 1743–1813.[PubMed]
    [Google Scholar]
  64. Yassin A. F., Rainey F. A., Brzezinka H., Jahnke K.-D., Weissbrodt H., Budzikiewicz H., Stackebrandt E., Schaal K. P. ( 1995 ). Lentzea gen. nov., a new genus of the order Actinomycetales . . Int J Syst Bacteriol 45, 357–363. [View Article] [PubMed]
    [Google Scholar]
  65. Yuan L.-J., Zhang Y.-Q., Yu L.-Y., Liu H.-Y., Guan Y., Lee J.-C., Kim C.-J., Zhang Y.-Q. ( 2010 ). Alloactinosynnema album gen. nov., sp. nov., a member of the family Actinosynnemataceae isolated from soil. . Int J Syst Evol Microbiol 60, 39–43. [View Article] [PubMed]
    [Google Scholar]
  66. Zhi X. Y., Li W. J., Stackebrandt E. ( 2009 ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59, 589–608. [View Article] [PubMed]
    [Google Scholar]
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Thermotunica guangxiensis gen. nov., sp. nov., isolated from mushroom residue compost
Int J Syst Evol Microbiol 64, 1593 (2014); https://doi.org/10.1099/ijs.0.057562-0
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