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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_5

gen. nov., sp. nov., isolated from mushroom residue compost Free

Abstract

A novel thermophilic actinomycete, designated AG2-7, was isolated from mushroom residue compost in Guangxi University, Nanning, China. The strain grew optimally at 45–60 °C, at pH 7.0 and with 0–3.0 % (w/v) NaCl. Vegetative mycelia were branched and whitish to pale yellow without fragmentation. Aerial mycelium was abundant, whitish and differentiated into long chains of spores, with a membranous structure or tunica partially covering the surface of aerial hyphae. The non-motile spores were oval in shape with a ridged surface. Strain AG-27 contained -diaminopimelic acid as the diagnostic diamino acid, and the whole-cell sugars were galactose and ribose. Major fatty acids were iso-C (27.51 %), iso-C (10.47 %) and anteiso-C (12.01 %). MK-9(H) was the predominant menaquinone. The polar phospholipids were diphosphatidylglycerol, ninhydrin-positive glycophospholipid, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylethanolamine, phosphatidylmethylethanolamine, an unknown phospholipid and unknown glucosamine-containing phospholipids. The G+C content of the genomic DNA was 63.6 mol%. 16S rRNA gene sequence analysis showed that the organism belonged to the family , suborder and showed more than 5 % divergence from other members of the family. Based on the phenotypic and phylogenetic data, strain AG2-7 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is AG2-7 ( = ATCC BAA-2499 = CGMCC 4.7099).

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Funding
This study was supported by the:
  • Guangxi Natural Science Foundation Key Programs (Award 2010GXNSFD013027)
  • National Natural Science Foundation of China (Award 30860006)
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2014-05-01
2026-01-20

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Thermotunica guangxiensis gen. nov., sp. nov., isolated from mushroom residue compost
Int J Syst Evol Microbiol 64, 1593 (2014); https://doi.org/10.1099/ijs.0.057562-0
/content/journal/ijsem/10.1099/ijs.0.057562-0
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