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Volume 64, Issue Pt_3

gen. nov., sp. nov., an anaerobic, filamentous bacterium of the phylum isolated from subseafloor sediment Free

Abstract

A novel, anaerobic filamentous bacterium, strain MO-CFX1, was isolated from a methanogenic community, which was originally established from subseafloor sediments collected from off the Shimokita Peninsula, Japan. Cells were non-spore-forming, non-motile, Gram-stain-negative and filamentous. The filaments were longer than 10 µm and 130–150 nm in width. Growth of the strain was observed at 10–37 °C (optimum 25–30 °C), at pH 5.5–8.5 (optimum pH 7.0) and in 0–50 g NaCl l (optimum 15 g NaCl l). The strain was able to grow with a number of carbohydrates in the presence of yeast extract. The major cellular fatty acids were monounsaturated Cω9, Cω7 and saturated C and C. The intact polar lipids of the strain were dominated by diacylglyceride and sphingolipid core lipid structures with monoglycosidic, mixed phosphomonoglycosidic and fatty-acid-modified monoglycosidic polar head groups. The G+C content of the genomic DNA was 52.4 mol%. Based on the comparative 16S rRNA gene sequence analysis, strain MO-CFX1 was affiliated with the class within the phylum and was most closely related to YMTK-2 (sequence identity of 91.0 %). Based on phenotypic and genetic properties of the novel isolate, we propose a novel species representing a new genus gen. nov., sp. nov., for strain MO-CFX1 ( = JCM 17238, = KCTC 5975). This is the first formal description, to our knowledge, of an isolate of the phylum from the deep-sea sedimentary environment.

  • Published Online:
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science
  • Ministry of Education, Culture, Sports, Science and Technology, Japan
  • European Research Council (ERC)
  • European Union’s Seventh Framework Programme–“Ideas” Specific Programme (Award 247153)
© 2014 IUMS
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2014-03-01
2024-04-16
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Pelolinea submarina gen. nov., sp. nov., an anaerobic, filamentous bacterium of the phylum Chloroflexi isolated from subseafloor sediment
Int J Syst Evol Microbiol 64, 812 (2014); https://doi.org/10.1099/ijs.0.057547-0
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