@article{mbs:/content/journal/ijsem/10.1099/ijs.0.057539-0, author = "Vaddavalli, Radha and Peddi, Sneha and Kothagauni, Srilekha Yadav and Begum, Zareena and Gaddam, Bhagyanarayana and Periketi, Madhusudhanachary and Linga, Venkateswar Rao", title = "Saccharopolyspora indica sp. nov., an actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_5", pages = "1559-1565", doi = "https://doi.org/10.1099/ijs.0.057539-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.057539-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel actinomycete strain, designated VRC122T, was isolated from a Callistemon citrinus rhizosphere sample collected from New Delhi, India, and its taxonomic status was determined by using a polyphasic approach. Strain VRC122T was a Gram-stain-positive, aerobic, non-motile, non-acid–alcohol-fast strain. Phylogenetic analysis based on 16S rRNA gene sequences showed the strain was placed in a well-separated sub-branch within the genus Saccharopolyspora . The highest levels of 16S rRNA gene sequence similarity were found with Saccharopolyspora hirsuta subsp. kobensis JCM 9109T (98.71 %), Saccharopolyspora antimicrobica I05-00074T (98.69 %) and Saccharopolyspora jiangxiensis W12T (98.66 %); 16S rRNA gene sequence similarities with type strains of all other species of the genus Saccharopolyspora were below 98 %. Chemosystematic studies revealed that it contained meso-diaminopimelic acid. Arabinose and galactose were the predominant whole-cell sugars. Diagnostic polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylcholine. MK-9(H6) was the predominant menaquinone. C14 : 0, C16 : 0, iso-C15 : 0, iso-C16 : 0, iso-C17 : 0, anteiso-C15 : 0, anteiso-C17 : 0, C17 : 0 cyclo and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) were the major cellular fatty acids. The G+C content of the genomic DNA was 69.5 mol%. The results of DNA–DNA hybridization (30 %, 22 % and 25 %, respectively) with type strains of the above-mentioned species, in combination with differences in physiological and biochemical data supported that strain VRC122T represents a novel species of the genus Saccharopolyspora , for which the name Saccharopolyspora indica sp. nov., is proposed. The type strain is VRC122T ( = KCTC 29208T = MTCC 11564T = MCC 2206T = ATCC BAA-2551T).", }