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Abstract

A Gram-stain-negative, non-spore-forming, non-flagellated, rod-shaped bacterial strain, SSK2-1, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea, and subjected to a polyphasic taxonomic study. Strain SSK2-1 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSK2-1 clustered with the type strains of two species, showing 96.5–96.8 % 16S rRNA gene sequence similarity. Strain SSK2-1 and DSM 13978 contained Q-10 as the predominant ubiquinone and Cω7 as the common major fatty acid. The polar lipid profile of strain SSK2-1 was similar to that of DSM 13978 by having phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid as the major components. The DNA G+C content of strain SSK2-1 was 60.1 mol%. Differential phenotypic properties, particularly temperature range for growth, oxidase activity and nitrate reduction, together with phylogenetic distinctiveness, revealed that strain SSK2-1 is separate from recognized species of the genus . On the basis of the data presented, strain SSK2-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SSK2-1 ( = KCTC 32535 = CECT 8397).

Funding
This study was supported by the:
  • , National Institute of Biological Resources (NIBR)
  • , Ministry of Environment (MOE)
  • , Program for Collection, Management and Utilization of Biological Resources
  • , Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea
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2014-03-01
2020-07-10
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