%0 Journal Article %A Hamada, Moriyuki %A Shibata, Chiyo %A Ishida, Yuumi %A Tamura, Tomohiko %A Yamamura, Hideki %A Hayakawa, Masayuki %A Suzuki, Ken-ichiro %T Agromyces iriomotensis sp. nov. and Agromyces subtropicus sp. nov., isolated from soil %D 2014 %J International Journal of Systematic and Evolutionary Microbiology, %V 64 %N Pt_3 %P 833-838 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.057349-0 %I Microbiology Society, %X Three novel Gram-stain-positive bacteria, designated IY07-20T, IY07-56T and IY07-113, were isolated from soil samples from Iriomote Island, Okinawa, Japan, and their taxonomic positions were investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the three isolates were closely related to the members of the genus Agromyces , with similarity range of 95.6–98.7 %. The isolates contained l-2,4-diaminobutylic acid, d-alanine, d-glutamic acid and glycine in their peptidoglycans. The predominant menaquinone was MK-12 and the major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C contents were 70.9–72.9 mol%. The chemotaxonomic characteristics of the isolates matched those described for members of the genus Agromyces . The results of phylogenetic analysis and DNA–DNA hybridization, along with differences in phenotypic characteristics between strains IY07-20T, IY07-56T and IY07-113 and the species of the genus Agromyces with validly published names, indicate that the three isolates merit classification as representatives of two novel species of the genus Agromyces , for which the names Agromyces iriomotensis sp. nov. and Agromyces subtropica sp. nov. are proposed; the type strains are IY07-20T ( = NBRC 106452T = DSM 26155T) and IY07-56T ( = NBRC 106454T = DSM 26153T), respectively. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.057349-0