1887

Abstract

A psychrotolerant methanogenic strain, X-18, was isolated from the soil of the Madoi wetland at Qinghai, Tibetan plateau, China. Cells were wavy rods (11–62 µm long) with blunt tapered ends and Gram-stain-negative. Strain X-18 grew strictly anaerobically and produced methane exclusively from H/CO. Growth occurred in the temperature range of 4–32 °C and optimally at 25 °C. Growth pH ranged from 6.5 to 8.0 and the optimum was 7.0. The G+C content of the genomic DNA of strain X-18 was 44.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and the alpha subunit of methyl-coenzyme M reductase indicated that strain X-18 was affiliated to the genus and was most closely related to Ki8-1, with 96.3 % 16S rRNA gene sequence similarity. However, strain X-18 could be distinguished from the existing species of the genus by its lower growth temperature and obligate hydrogenotrophic methanogenesis. On the basis of phenotypic characteristics and phylogenetic analysis, strain X-18 represents a novel species of the genus , for which the name sp. nov. is proposed and strain X-18 is assigned as the type strain ( = CGMCC 1.5186 = JCM 19216).

Funding
This study was supported by the:
  • , National Science Foundation of China , (Award 30621005 and 30830007)
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2014-02-01
2020-11-26
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