1887

Abstract

A novel sulfate-reducing bacterium designated strain BE2801 was isolated from oil-polluted estuarine sediments (Berre Lagoon, France). Cells were Gram-stain-negative, motile, slightly curved or vibrioid rods. Optimal growth of strain BE2801 occurred at 30–32 °C, 0.5–1.5% NaCl (w/v) and pH 7.2–7.4. Strain BE2801 grew with C to C fatty acids or C to C -alkenes as electron donors. Acetate and carbon dioxide were the oxidation products. The major cellular fatty acids were C, Cω7 and Cω7. The DNA G+C content was 50.2 mol%. 16S rRNA and gene sequence analysis indicated that strain BE2801 was a member of the family within the class . DNA–DNA hybridization with the most closely related taxon demonstrated 14.8 % relatedness. Based on phenotypic and phylogenetic evidence, strain BE2801 ( = DSM 25524 = JCM 18157) is proposed to be a representative of a novel species of the genus , for which the name sp. nov. is suggested.

Funding
This study was supported by the:
  • , CNRS-INSU , (Award BIOHYDEX EC2CO)
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2014-02-01
2020-09-29
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References

  1. Buck J. D. ( 1982 ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44, 992993.[PubMed]
    [Google Scholar]
  2. Cline J. D. ( 1969 ). Spectrophotometric determination of hydrogen sulfide in natural waters. . Limnol Oceanogr 14, 454458. [CrossRef]
    [Google Scholar]
  3. Cravo-Laureau C., Matheron R., Cayol J.-L., Joulian C., Hirschler-Réa A. ( 2004a ). Desulfatibacillum aliphaticivorans gen. nov., sp. nov., an n-alkane- and n-alkene-degrading, sulfate-reducing bacterium. . Int J Syst Evol Microbiol 54, 7783. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cravo-Laureau C., Matheron R., Joulian C., Cayol J.-L., Hirschler-Réa A. ( 2004b ). Desulfatibacillum alkenivorans sp. nov., a novel n-alkene-degrading, sulfate-reducing bacterium, and emended description of the genus Desulfatibacillum . . Int J Syst Evol Microbiol 54, 16391642. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cravo-Laureau C., Labat C., Joulian C., Matheron R., Hirschler-Réa A. ( 2007 ). Desulfatiferula olefinivorans gen. nov., sp. nov., a long-chain n-alkene-degrading, sulfate-reducing bacterium. . Int J Syst Evol Microbiol 57, 26992702. [CrossRef] [PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [CrossRef] [PubMed]
    [Google Scholar]
  7. Escara J. F., Hutton J. R. ( 1980 ). Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. . Biopolymers 19, 13151327. [CrossRef] [PubMed]
    [Google Scholar]
  8. Grossi V., Cravo-Laureau C., Guyoneaud R., Ranchou-Peyruse A., Hirschler-Réa A. ( 2008 ). Metabolism of n-alkanes and n-alkenes by anaerobic bacteria: a summary. . Org Geochem 39, 11971203. [CrossRef]
    [Google Scholar]
  9. Grossi V., Cravo-Laureau C., Rontani J.-F., Cros M., Hirschler-Réa A. ( 2011 ). Anaerobic oxidation of n-alkenes by sulphate-reducing bacteria from the genus Desulfatiferula: n-ketones as potential metabolites. . Res Microbiol 162, 915922. [CrossRef] [PubMed]
    [Google Scholar]
  10. HuB V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [CrossRef] [PubMed]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  12. Rabus R., Hansen T. A., Widdel F. ( 2006 ). Dissimilatory sulfate- and sulfur-reducing prokaryotes. . In The Prokaryotes: a Handbook on the Biology of Bacteria, vol. 2, Ecophysiology and Biochemistry, pp.659768. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. . New York:: Springer;.
    [Google Scholar]
  13. Vogel A. I. ( 1961 ). A Text Book of Quantitative Inorganic Analysis. London:: Longmans;.
    [Google Scholar]
  14. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  15. Widdel F., Bak F. ( 1992 ). Gram-negative mesophilic sulfate-reducing bacteria. . In The Prokaryotes, vol. 4, pp. 33523378. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;. [CrossRef]
    [Google Scholar]
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