1887

Abstract

A Gram-stain-positive, rod-shaped, facultatively anaerobic bacterium, designated strain ES3-24, was isolated from a selenium mineral soil. The isolate was endospore-forming, nitrate-reducing and motile by means of peritrichous flagella. The major menaquinone was menaquinone 7 (MK-7) and the predominant fatty acids (>5 %) were anteiso-C, iso-C, C and anteiso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and two unknown aminophospholipids. Strain ES3-24 contained -diaminopimelic acid in the cell-wall peptidoglycan and the DNA G+C content was 49.6 mol%. According to phylogenetic analysis based on the 16S rRNA gene sequence, strain ES3-24 was most closely related to A35, with 16S rRNA gene sequence identity of 98.3 %, while the other members of the genus had 16S rRNA gene sequence identities of less than 95.0 %. DNA–DNA relatedness between strain ES3-24 and CCTCC AB206026 was 39.3 %. In addition, strain ES3-24 showed obvious differences from closely related species in major polar lipids, nitrate reduction and other physiological and biochemical characteristics. The data from our polyphasic taxonomic study reveal that strain ES3-24 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ES3-24 ( = KCTC 33157 = CCTCC AB2013097).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 41171213)
  • , Tobacco Company of Enshi, Hubei Province, PR China
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2014-03-01
2020-05-26
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