1887

Abstract

A novel aerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-stain-negative, coccoid to short-rod-shaped bacterial strain, designated YW11, was isolated from soil under long-term application of triazophos. The strain was able to hydrolyse triazophos. Strain YW11 grew at 15–40 °C (optimum at 28 °C), at pH 5.0–8.0 (optimum at pH 7.5) and with 0–5.0 % (w/v) NaCl (optimum at 0.5 %). The major respiratory quinone was ubiquinone 10 (Q-10) and the major cellular fatty acids were Cω7, C, C 2-OH and C. The genomic DNA G+C content of strain YW11 was 69.6±0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unknown glycolipid and two unknown aminolipids. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain YW11 was a member of the genus , and showed the highest sequence similarity to KACC 11686 (97.9 %) and KACC 19645 (97.8 %) and then to KACC 13843 (96.9 %). Strain YW11 showed low DNA–DNA relatedness with KACC 11686 (32.3±2.9 %), KACC 16549 (28.2±2.6 %) and KACC 13843 (30.2±2.6 %). Based on the results of phylogenetic analysis and DNA–DNA hybridization, the whole-cell fatty acid composition as well as biochemical characteristics, strain YW11 was clearly distinguished from all recognized species of the genus and should be assigned to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YW11 ( = KACC 17225 = CCTCC AB2013041).

Funding
This study was supported by the:
  • , National Science and Technology Support Plan , (Award 2012BAD15B03 and 2013AA102804C)
  • , National Natural Science Foundation of China , (Award 31270157)
  • , Fundamental Research Funds for the Central Universities , (Award KYZ201122)
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2014-04-01
2020-08-04
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