1887

Abstract

A Gram-stain-positive, rod-shaped, spore-forming and strictly anaerobic bacterium, designated UB-B.2, was isolated from an industrial effluent anaerobic digester sample. It grew optimally at 30 °C and pH 7.0. Comparative analysis of the 16S rRNA gene sequence confirmed that strain UB-B.2 was closely related to DSM 13479 (97.84 % similarity), a member of rRNA gene cluster XIVa of the genus , and formed a coherent cluster with other related members of the ( ) rRNA group in phylogenetic analyses. The end products of glucose fermentation by strain UB-B.2 were acetate and propionate. The G+C content of the DNA was 51.4 mol%. Although strain UB-B.2 showed 97.8 % 16S rRNA gene sequence identity to the type strain of , it exhibited only 38.4 % relatedness at the whole-genome level. It also showed differences from its closest phylogenetic relative, DSM 13479, in phenotypic characteristics such as hydrolysis of aesculin, starch and urea and fermentation end products. Both strains showed phenotypic differences from the members of rRNA gene cluster XIVa of the genus . Based on these differences, DSM 13479 and strain UB-B.2 were identified as representatives of a new genus of the family . Thus, we propose the reclassification of as gen. nov., comb. nov., the type species of the new genus (type strain DSM 13479 = CCUG 43506 = MTCC 10951). Strain UB-B.2 ( = MTCC 11101 = DSM 24995) is assigned to the novel species gen. nov., sp. nov as the type strain.

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2014-03-01
2019-10-21
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