1887

Abstract

A polyphasic study was undertaken to clarify the taxonomic position of strains isolated from Atlantic salmon () cage-farmed in Chile. Four salmon and three seal isolates showed minor differences in the SDS-PAGE protein analysis. Thus, a major protein band present in the salmon isolates, of approximately 22.4 kDa, was absent in the pinniped strains, regardless of the growth media employed. In addition, the pinniped strains showed protein bands with molecular masses of 71.5 and 14.2 kDa, when grown on trypticase soy agar supplemented with 1 % NaCl, or 25.6 kDa, when grown on Columbia blood agar, not present in the Atlantic salmon strains. A high similarity in the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS spectra of the strains was observed, although some minor peaks were absent in the fish isolates. Fatty acid methyl esters from isolates with different host origin significantly (<0.05) differed in the content of C, C, Cω9, Cω6,9,12,15 and summed features 3, 5 and 8. The salmon isolates formed a separate cluster in the phylogenetic analysis of housekeeping genes, separately or as concatenated sequences. Sequence divergences among salmon and seal strains were in the range of inter-subspecies differentiation for (2.5 %), (1.8 %), (2.1 %), (1.7 %) and (2.0 %) genes. DNA–DNA hybridization results confirmed those of sequencing, showing reassociation values between seal and salmon strains close to the borderline of species definition. Differences in growth at low temperatures and in the haemolytic capacities were also observed between both groups of isolates. On the basis of all these results, the salmon isolates represent a novel subspecies of , for which the name subsp. subsp. nov. is proposed. The type strain is C-4 ( = CECT 7921 = DSM 24768). The subspecies subsp. subsp. nov. is automatically created. An emended description of is also provided.

Funding
This study was supported by the:
  • Comisión Nacional de Investigación Científica y Tecnológica–CONICYT (Chile) (Award FONDECYT 1090054)
  • Ministerio de Ciencia e Innovación-MICINN (Spain) (Award AGL2010-18438)
  • Ministerio de Ciencia e Innovación-MICINN (Spain)
  • Comisión Nacional de Investigación Científica y Tecnológica–CONICYT (Chile) (Award CONICYT/FONDAP/15110027)
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2014-05-01
2024-03-29
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