1887

Abstract

Two halophilic archaeal strains, R2 and R27, were isolated from the brown alga produced at Dalian, Liaoning Province, China. Both had pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They grew optimally at 42 °C, pH 7.0 and in the presence of 3.1–3.4 M NaCl and 0.03–0.5 M Mg. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and four major glycolipids chromatographically identical to those of JCM 11222. 16S rRNA gene analysis revealed that each strain had two dissimilar 16S rRNA genes and both strains were phylogenetically related to JCM 11222 (92.7–98.8 % similarities). The gene similarities between strains R2 and R27 and between these strains and JCM 11222 were 95.7 %, 96.1 % and 95.8 %, respectively. The DNA G+C contents of strains R2 and R27 were 62.5 mol% and 64.0 mol%, respectively. The DNA–DNA hybridization values between strains R2 and R27 and between the two strains and JCM 11222 were 43 %, 52 % and 47 %, respectively. It was concluded that strain R2 ( = CGMCC 1.10586 = JCM 17263) and strain R27 ( = CGMCC 1.10591 = JCM 17268) represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. An emended description of the genus is also presented.

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31370054)
  • , China Ocean Mineral Resources R & D Association (COMRA) Special Foundation , (Award DY125-15-R-03)
  • , Qinglan Project of Jiangsu Province
  • , Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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/content/journal/ijsem/10.1099/ijs.0.056887-0
2014-01-01
2020-11-30
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