1887

Abstract

Two halophilic archaeal strains, R2 and R27, were isolated from the brown alga produced at Dalian, Liaoning Province, China. Both had pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They grew optimally at 42 °C, pH 7.0 and in the presence of 3.1–3.4 M NaCl and 0.03–0.5 M Mg. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and four major glycolipids chromatographically identical to those of JCM 11222. 16S rRNA gene analysis revealed that each strain had two dissimilar 16S rRNA genes and both strains were phylogenetically related to JCM 11222 (92.7–98.8 % similarities). The gene similarities between strains R2 and R27 and between these strains and JCM 11222 were 95.7 %, 96.1 % and 95.8 %, respectively. The DNA G+C contents of strains R2 and R27 were 62.5 mol% and 64.0 mol%, respectively. The DNA–DNA hybridization values between strains R2 and R27 and between the two strains and JCM 11222 were 43 %, 52 % and 47 %, respectively. It was concluded that strain R2 ( = CGMCC 1.10586 = JCM 17263) and strain R27 ( = CGMCC 1.10591 = JCM 17268) represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. An emended description of the genus is also presented.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31370054)
  • China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY125-15-R-03)
  • Qinglan Project of Jiangsu Province
  • Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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/content/journal/ijsem/10.1099/ijs.0.056887-0
2014-01-01
2024-03-28
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References

  1. Boucher Y., Douady C. J., Sharma A. K., Kamekura M., Doolittle W. F. ( 2004 ). Intragenomic heterogeneity and intergenomic recombination among haloarchaeal rRNA genes. . J Bacteriol 186, 39803990. [View Article] [PubMed]
    [Google Scholar]
  2. Burns D. G., Janssen P. H., Itoh T., Kamekura M., Li Z., Jensen G., Rodríguez-Valera F., Bolhuis H., Dyall-Smith M. L. ( 2007 ). Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain. . Int J Syst Evol Microbiol 57, 387392. [View Article] [PubMed]
    [Google Scholar]
  3. Colwell R. R., Litchfield C. D., Vreeland R. H., Kiefer L. A., Gibbons N. E. ( 1979 ). Taxonomic studies of red halophilic bacteria. . Int J Syst Bacteriol 29, 379399. [View Article]
    [Google Scholar]
  4. Cui H.-L., Zhou P.-J., Oren A., Liu S.-J. ( 2009 ). Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium . . Extremophiles 13, 3137. [View Article] [PubMed]
    [Google Scholar]
  5. Cui H.-L., Gao X., Yang X., Xu X.-W. ( 2010 ). Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. . Extremophiles 14, 493499. [View Article] [PubMed]
    [Google Scholar]
  6. Cui H.-L., Yang X., Mou Y. Z. ( 2011 ). Salinarchaeum laminariae gen. nov., sp. nov.: a new member of the family Halobacteriaceae isolated from salted brown alga Laminaria . . Extremophiles 15, 625631. [View Article] [PubMed]
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  8. Huß V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  9. Kocur M., Hodgkiss W. ( 1973 ). Taxonomic status of the genus Halococcus Schoop. . Int J Syst Bacteriol 23, 151156. [View Article]
    [Google Scholar]
  10. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [View Article] [PubMed]
    [Google Scholar]
  11. Minegishi H., Kamekura M., Itoh T., Echigo A., Usami R., Hashimoto T. ( 2010 ). Further refinement of the phylogeny of the Halobacteriaceae phylogeny based on the full-length RNA polymerase subunit B (rpoB′) gene. . Int J Syst Evol Microbiol 60, 23982408. [View Article] [PubMed]
    [Google Scholar]
  12. Oren A., Ventosa A., Grant W. D. ( 1997 ). Proposed minimal standards for description of new taxa in the order Halobacteriales . . Int J Syst Bacteriol 47, 233238. [View Article]
    [Google Scholar]
  13. Owen R. J., Pitcher D. ( 1985 ). Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. . In Chemical Methods in Bacterial Systematics, pp. 6793. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  14. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  16. Vreeland R. H., Straight S., Krammes J., Dougherty K., Rosenzweig W. D., Kamekura M. ( 2002 ). Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. . Extremophiles 6, 445452. [View Article] [PubMed]
    [Google Scholar]
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