@article{mbs:/content/journal/ijsem/10.1099/ijs.0.056812-0, author = "Du, Zong-Jun and Wang, Ying and Dunlap, Christopher and Rooney, Alejandro P. and Chen, Guan-Jun", title = "Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_5", pages = "1690-1696", doi = "https://doi.org/10.1099/ijs.0.056812-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.056812-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "The taxonomic characteristics of two bacterial strains, FH5T and SS4, isolated from enrichment cultures obtained from two distinct marine environments, were determined. These bacteria were Gram-stain-negative, facultatively anaerobic rods. Growth occurred at 20–40 °C (optimum, 28–32 °C), pH 5.5–9.0 (optimum, pH 7.0–7.5) and in the presence of 1–7 % NaCl (optimum, 2–4 %). The major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0. Menaquinone 7 (MK-7) was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, an unkown phospholipid and an unknown lipid. The DNA G+C contents of strains FH5T and SS4 were both determined to be 42.0 mol%. The results of DNA–DNA hybridization studies indicated that the FH5T and SS4 genomes share greater than 95 % relatedness. The strains formed a distinct phyletic line within the class Bacteroidia , with less than 89.4 % sequence similarity to their closest relatives with validly published names. On the basis of physiological and biochemical characteristics, 16S rRNA gene sequences and chemical properties, a novel genus and species, Draconibacterium orientale gen. nov., sp. nov., within the class Bacteroidia , are proposed, with strain FH5T ( = DSM 25947T = CICC 10585T) as the type strain. In addition, a new family, Draconibacteriaceae fam. nov., is proposed to accommodate Draconibacterium gen. nov.", }