1887

Abstract

A bacterium, designated XC21-2, was isolated from a saline-alkaline soil sample from China. Cells were Gram-stain-negative, rod-shaped and motile and grew optimally at 35–37 °C, pH 6.0–7.0 and in the presence of 0.5 % (w/v) NaCl. Growth occurred in the range pH 5.5–9.0 and in the presence of up to 4 % (w/v) NaCl. The major cellular fatty acids were iso-C, iso-C and iso-Cω9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an uncharacterized amino-group-containing polar lipid. The major quinone was ubiquinone 8 (Q-8) and the G+C content of the genomic DNA was 66.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XC21-2 formed a tight phylogenetic lineage with KCTC 12543 within the genus and was most closely related to KCTC 12543 and ATCC 700993, with 97.9 and 97.5 % 16S rRNA gene sequence similarity, respectively. On the basis of the unique physiological profile of the isolate and its phylogenetic divergence from known species, strain XC21-2 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is XC21-2 ( = CGMCC 1.10978 = KCTC 23877).

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2014-03-01
2019-10-20
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