1887

Abstract

A novel Gram-stain-positive, non-spore-forming, pale yellow, irregular rod-shaped bacterium designated strain HWE2-01 was isolated from the surface-sterilized root of horseweed (). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HWE2-01 belongs to the family and showed sequence similarity levels of 97.1–97.7 % with species of the genus , 95.9–97.6 % with species of the genus and 97.1 % with . The highest sequence similarity (97.7 %) was with 0543. The genomic DNA G+C content of the novel strain was 68.1 mol%. The predominant cellular fatty acid of strain HWE2-01 was anteiso-C, major menaquinones were MK-10, MK-9 and MK-11, and diagnostic polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The peptidoglycan of the novel strain contained 2,4-diaminobutyric acid, alanine, glycine and glutamic acid. The cell-wall sugars of strain HWE2-01 were galactose, mannose and rhamnose. The murein was of the acetyl type. Based on the results of the phenotypic and phylogenetic analysis, strain HWE2-01 differed in some respects from other members of the family . Therefore, strain HWE2-01 is proposed to represent a novel species of a new genus in the family with the name gen. nov., sp. nov. (type strain = HWE2-01 = KCTC 29231 = JCM 19257).

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2014-08-01
2019-10-18
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