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Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, motile by gliding and rod-shaped bacterial strain, RSSK-12, was isolated from the zone where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain RSSK-12 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSSK-12 clustered with the type strains of two species, with which it exhibited 94.8–96.9 % sequence similarity. Sequence similarities to the type strains of species of the genus and of other recognized species were 94.7–95.5 % and less than 91.9 %, respectively. Strain RSSK-12 contained MK-6 as the predominant menaquinone and iso-C and iso-C 3-OH as the major fatty acids. The major polar lipids of strain RSSK-12 were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain RSSK-12 was 38.9 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, revealed that strain RSSK-12 is separate from other species. On the basis of the data presented, strain RSSK-12 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RSSK-12 ( = KCTC 32536 = CECT 8398).

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2014-02-01
2020-01-28
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