@article{mbs:/content/journal/ijsem/10.1099/ijs.0.056689-0, author = "Li, Aihua and Liu, Hongcan and Sun, Bingda and Zhou, Yuguang and Xin, Yuhua", title = "Flavobacterium lacus sp. nov., isolated from a high-altitude lake, and emended description of Flavobacterium filum", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_3", pages = "933-939", doi = "https://doi.org/10.1099/ijs.0.056689-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.056689-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated NP180T and NR80, were isolated from water of Nam Co Lake, located in Tibet, China. Growth of strains NP180T and NR80 occurred at 4–25 °C and at pH 6.5–10.0 (optima, 15–20 °C and pH 7.5–8.5). The 16S rRNA gene sequence similarity to the phylogenetically closest related strains, Flavobacterium filum EMB 34T, F. ponti GSW-R14T and F. gelidilacus LMG 21477T, was 95.1, 94.8 and 94.6 %, respectively. The predominant fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0). The major menaquinone of the two strains was menaquinone 6 (MK-6). Phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid were the major polar lipids in both strains. The G+C contents of the genomic DNA were 34.9 and 35.1 mol%, respectively, for strains NP180T and NR80. DNA–DNA relatedness between strains NP180T and NR80 was 99 %, indicating that they belong to the same species. According to phylogenetic inference and phenotypic characteristics, a novel species, Flavobacterium lacus sp. nov., is proposed. The type strain is NP180T ( = CGMCC 1.12504T = NBRC 109715T). An emended description of Flavobacterium filum is also provided.", }