1887

Abstract

Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated NP180 and NR80, were isolated from water of Nam Co Lake, located in Tibet, China. Growth of strains NP180 and NR80 occurred at 4–25 °C and at pH 6.5–10.0 (optima, 15–20 °C and pH 7.5–8.5). The 16S rRNA gene sequence similarity to the phylogenetically closest related strains, EMB 34, GSW-R14 and LMG 21477, was 95.1, 94.8 and 94.6 %, respectively. The predominant fatty acids were iso-C, iso-C G, iso-C 3-OH and summed feature 9 (comprising iso-Cω9 and/or 10-methyl C). The major menaquinone of the two strains was menaquinone 6 (MK-6). Phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid were the major polar lipids in both strains. The G+C contents of the genomic DNA were 34.9 and 35.1 mol%, respectively, for strains NP180 and NR80. DNA–DNA relatedness between strains NP180 and NR80 was 99 %, indicating that they belong to the same species. According to phylogenetic inference and phenotypic characteristics, a novel species, sp. nov., is proposed. The type strain is NP180 ( = CGMCC 1.12504 = NBRC 109715). An emended description of is also provided.

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2014-03-01
2019-10-22
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