1887

Abstract

A thermophilic bacterium, designated DX-1, was isolated from the anode biofilm of a microbial fuel cell (MFC). Cells of strain DX-1 were oxidase-positive, catalase-positive and Gram-staining-negative. The strain was found to be rod-shaped and non-motile and to produce subterminal spores. The strain was able to grow with NaCl at concentrations ranging from 0 to 6 %, at temperatures of 25–60 °C (optimum 55 °C) and pH 6.0–8.0 (optimum pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DX-1 formed a cluster with DSM 10633 (96.9 % 16S rRNA sequence similarity), DSM 17951 (95.8 %), DSM 17952 (95.7 %) and DSM 12654 (95.3 %). The G+C content of the genomic DNA was 40.4 mol%. The major quinone was MK-7, the peptidoglycan type was -Lys←-Asp, and the major cellular fatty acids (>5 %) were iso-C and iso-C. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and phospholipids of unknown composition. Based on phenotypic characteristics, chemotaxonomic features and results of phylogenetic analyses, the strain was determined to represent a distinct novel species of the genus , and the name proposed for the novel species is sp. nov., with type strain DX-1 ( = CGMCC 1.12358 = KCTC 33127).

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2014-05-01
2019-10-16
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