1887

Abstract

A Gram-negative, proteorhodopsin-containing, orange pigmented, rod-shaped and strictly aerobic bacterium, designated strain AKS622, was isolated from a glacier core collected from the coast of King George Island, Antarctica. 16S rRNA gene sequence analysis revealed that strain AKS622 was affiliated to the genus of the family and showed highest similarity to S1-08 (97.9 %). The level of DNA–DNA relatedness between strain AKS622 and S1-08 was 46 %. Optimal growth of strain AKS622 was observed at pH 7.0, at 15 °C and with 2.0 % NaCl. The predominant cellular fatty acids were anteiso-C, iso-C, iso-C 3-OH, C 2-OH and summed feature 3 (comprising Cω7 and/or Cω6). The DNA G+C content was 37.9 mol%. The major respiratory quinone was MK-6. Phosphatidylethanolamine, four unidentified glycolipids, three unidentified aminolipids and one unidentified lipid were detected as major polar lipids. On the basis of the data from this polyphasic taxonomic study, it was concluded that strain AKS622 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is AKS622 ( = KCCM 43019 = JCM 14068). Emended descriptions of Park 2012 and Yi and Chun 2012 are given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.056606-0
2014-02-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/2/400.html?itemId=/content/journal/ijsem/10.1099/ijs.0.056606-0&mimeType=html&fmt=ahah

References

  1. Barbeyron T. , Lerat Y. , Sassi J. F. , Le Panse S. , Helbert W. , Collén P. N. . ( 2011; ). Persicivirga ulvanivorans sp. nov., a marine member of the family Flavobacteriaceae that degrades ulvan from green algae. . Int J Syst Evol Microbiol 61:, 1899–1905. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bernardet J.-F. , Nakagawa Y. . ( 2006; ). An introduction to the family Flavobacteriaceae . . In The Prokaryotes, a Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 455–480. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . . New York:: Springer;.
    [Google Scholar]
  3. Bernardet J.-F. , Segers P. , Vancanneyt M. , Berthe F. , Kersters K. , Vandamme P. . ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol 46:, 128–148. [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F. , Nakagawa Y. , Holmes B. . ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  5. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  6. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  7. Garrity G. M. , Holt J. G. . ( 2001; ). The road map to the Manual . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 1, pp. 119–166. Edited by Boone D. R. , Castenholz R. W. , Garrity G. M. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  8. Jooste, P. J. (1985). The taxonomy and significance of Flavobacterium–Cytophaga strains from daily sources. PhD thesis, University of the Orange Free State, Bloemfontein, South Africa.
  9. Khan S. T. , Nakagawa Y. , Harayama S. . ( 2006; ). Sandarakinotalea sediminis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae . . Int J Syst Evol Microbiol 56:, 959–963. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kwon K. K. , Lee H. S. , Yang S.-H. , Kim S.-J. . ( 2005; ). Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’. . Int J Syst Evol Microbiol 55:, 2033–2037. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lau S. C. K. , Tsoi M. M. Y. , Li X. , Plakhotnikova I. , Dobretsov S. , Wong P. K. , Pawlik J. R. , Qian P. Y. . ( 2005; ). Nonlabens tegetincola gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a microbial mat in a subtropical estuary. . Int J Syst Evol Microbiol 55:, 2279–2283. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lau S. C. , Tsoi M. M. , Li X. , Plakhotnikova I. , Dobretsov S. , Wu M. , Wong P. K. , Pawlik J. R. , Qian P. Y. . ( 2006; ). Stenothermobacter spongiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a marine sponge in the Bahamas, and emended description of Nonlabens tegetincola . . Int J Syst Evol Microbiol 56:, 181–185. [CrossRef] [PubMed]
    [Google Scholar]
  14. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  15. Nedashkovskaya O. I. , Kwon K. K. , Kim S.-J. . ( 2009; ). Reclassification of Donghaeana dokdonensis Yoon et al. 2006 as Persicivirga dokdonensis comb. nov. and emended descriptions of the genus Persicivirga and of Persicivirga xylanidelens O’Sullivan et al. 2006. . Int J Syst Evol Microbiol 59:, 824–827. [CrossRef] [PubMed]
    [Google Scholar]
  16. O’Sullivan L. A. , Rinna J. , Humphreys G. , Weightman A. J. , Fry J. C. . ( 2006; ). Culturable phylogenetic diversity of the phylum ‘Bacteroidetes’ from river epilithon and coastal water and description of novel members of the family Flavobacteriaceae: Epilithonimonas tenax gen. nov., sp. nov. and Persicivirga xylanidelens gen. nov., sp. nov. . Int J Syst Evol Microbiol 56:, 169–180. [CrossRef] [PubMed]
    [Google Scholar]
  17. Park S. , Yoshizawa S. , Chiura H. X. , Muramatsu Y. , Nakagawa Y. , Kogure K. , Yokota A. . ( 2012; ). Nonlabens marinus [corrected] sp. nov., a novel member of the Flavobacteriaceae isolated from the Pacific Ocean. . Antonie van Leeuwenhoek 102:, 669–676. [CrossRef] [PubMed]
    [Google Scholar]
  18. Park S. , Kang C. H. , Yoon J. H. . ( 2013; ). Nonlabens arenilitoris sp. nov., a member of the family Flavobacteriaceae isolated from seashore sand. . Antonie van Leeuwenhoek 103:, 1125–1132. [CrossRef] [PubMed]
    [Google Scholar]
  19. Reichenbach H. . ( 1992; ). The order Cytophagales . . In The Prokaryotes, pp. 3631–3675. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . . New York:: Springer;.[CrossRef]
    [Google Scholar]
  20. Rosselló-Mora R. , Amann R. . ( 2001; ). The species concept for prokaryotes. . FEMS Microbiol Rev 25:, 39–67. [CrossRef] [PubMed]
    [Google Scholar]
  21. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  22. Seo H. J. , Bae S. S. , Lee J.-H. , Kim S.-J. . ( 2005; ). Photobacterium frigidiphilum sp. nov., a psychrophilic, lipolytic bacterium isolated from deep-sea sediments of Edison Seamount. . Int J Syst Evol Microbiol 55:, 1661–1666. [CrossRef] [PubMed]
    [Google Scholar]
  23. Smibert R. M. , Krieg N. R. . ( 1981; ). General characterization. . In Manual of Methods for General Bacteriology, pp. 409–443. Edited by Gerhardt P. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Phillips G. B. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  24. Sohn J. H. , Kwon K. K. , Kang J.-H. , Jung H.-B. , Kim S.-J. . ( 2004; ). Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. . Int J Syst Evol Microbiol 54:, 1483–1487. [CrossRef] [PubMed]
    [Google Scholar]
  25. Stackebrandt E. , Liesack W. . ( 1993; ). Nucleic acids and classification. . In Handbook of New Bacterial Systematics, pp. 158–160. Edited by Goodfellow M. , O’Donnell A. G. . . London:: Academic Press;.
    [Google Scholar]
  26. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  27. Yang S.-H. , Kwon K. K. , Lee H.-S. , Kim S.-J. . ( 2006; ). Shewanella spongiae sp. nov., isolated from a marine sponge. . Int J Syst Evol Microbiol 56:, 2879–2882. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yang S.-H. , Seo H.-S. , Oh H.-M. , Kim S.-J. , Lee J.-H. , Kwon K. K. . ( 2013; ). Brumimicrobium mesophilum sp. nov., isolated from a tidal flat sediment, and emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale . . Int J Syst Evol Microbiol 63:, 1105–1110. [CrossRef] [PubMed]
    [Google Scholar]
  29. Yi H. , Chun J. . ( 2012; ). Unification of the genera Nonlabens, Persicivirga, Sandarakinotalea and Stenothermobacter into a single emended genus, Nonlabens, and description of Nonlabens agnitus sp. nov.. Syst Appl Microbiol 35:, 150–155. [CrossRef] [PubMed]
    [Google Scholar]
  30. Yoon J.-H. , Kang S.-J. , Lee C.-H. , Oh T.-K. . ( 2006; ). Donghaeana dokdonensis gen. nov., sp. nov., isolated from sea water. . Int J Syst Evol Microbiol 56:, 187–191. [CrossRef] [PubMed]
    [Google Scholar]
  31. Yoshizawa S. , Kawanabe A. , Ito H. , Kandori H. , Kogure K. . ( 2012; ). Diversity and functional analysis of proteorhodopsin in marine Flavobacteria. . Environ Microbiol 14:, 1240–1248. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.056606-0
Loading
/content/journal/ijsem/10.1099/ijs.0.056606-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error