%0 Journal Article %A Miao, Chaohua %A Jia, Fangfang %A Wan, Yusong %A Zhang, Wei %A Lin, Min %A Jin, Wujun %T Halomonas huangheensis sp. nov., a moderately halophilic bacterium isolated from a saline–alkali soil %D 2014 %J International Journal of Systematic and Evolutionary Microbiology, %V 64 %N Pt_3 %P 915-920 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.056556-0 %I Microbiology Society, %X A novel, Gram-stain-negative, aerobic, rod-shaped, non-motile and moderately halophilic bacterium, designated strain BJGMM-B45T, was isolated from a saline–alkali soil collected from Shandong Province, China. Growth of strain BJGMM-B45T occurred at 10–45 °C (optimum, 30 °C) and pH 5.0–12.0 (optimum, pH 7.0) on Luria–Bertani agar medium with 1–20 % (w/v) NaCl (optimum, 7–10 %). The predominant respiratory quinone was Q-9. The major cellular fatty acids (>5 %) were C18 : 1ω7c, C16 : 0, C19 : 0 cyclo ω8c, summed feature 3, C12 : 0 3-OH and C12 : 0. The genomic DNA G+C content was 57.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BJGMM-B45T belonged to the genus Halomonas in the class Gammaproteobacteria . The closest relatives were Halomonas cupida DSM 4740T (98.2 % 16S rRNA gene sequence similarity) and Halomonas denitrificans M29T (97.8 %). Levels of DNA–DNA relatedness between strain BJGMM-B45T and Halomonas cupida CGMCC 1.2312T and Halomonas denitrificans DSM 18045T were 57.0 and 58.9 %, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BJGMM-B45T is considered to represent a novel species of the genus Halomonas , for which the name Halomonas huangheensis sp. nov. is proposed. The type strain is BJGMM-B45T ( = ACCC 05850T = KCTC 32409T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.056556-0