Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_3

sp. nov., a moderately halophilic bacterium isolated from a saline–alkali soil Free

Abstract

A novel, Gram-stain-negative, aerobic, rod-shaped, non-motile and moderately halophilic bacterium, designated strain BJGMM-B45, was isolated from a saline–alkali soil collected from Shandong Province, China. Growth of strain BJGMM-B45 occurred at 10–45 °C (optimum, 30 °C) and pH 5.0–12.0 (optimum, pH 7.0) on Luria–Bertani agar medium with 1–20 % (w/v) NaCl (optimum, 7–10 %). The predominant respiratory quinone was Q-9. The major cellular fatty acids (>5 %) were Cω7, C, C cyclo ω8c, summed feature 3, C 3-OH and C. The genomic DNA G+C content was 57.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BJGMM-B45 belonged to the genus in the class . The closest relatives were DSM 4740 (98.2 % 16S rRNA gene sequence similarity) and M29 (97.8 %). Levels of DNA–DNA relatedness between strain BJGMM-B45 and CGMCC 1.2312 and DSM 18045 were 57.0 and 58.9 %, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BJGMM-B45 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BJGMM-B45 ( = ACCC 05850 = KCTC 32409).

  • Published Online:
Funding
This study was supported by the:
  • National Research and Development Project of Transgenic Crops of China (Award 2013ZX08012-001 and 2011ZX08012-001 )
  • National Natural Science Foundation of China (Award 31170105)
  • Ministry of Science and Technology of China
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.056556-0
2014-03-01
2025-03-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/3/915.html?itemId=/content/journal/ijsem/10.1099/ijs.0.056556-0&mimeType=html&fmt=ahah

References

  1. Anan’ina L. N., Plotnikova E. G., Gavrish E. Yu., Demakov V. A., Evtushenko L. I. ( 2007 ). Salinicola socius gen. nov., sp. nov., a moderately halophilic bacterium from a naphthalene-utilizing microbial association. . Microbiology 76, 369–376. [PubMed] [CrossRef]
    [Google Scholar]
  2. Arahal D. R., Castillo A. M., Ludwig W., Schleifer K. H., Ventosa A. ( 2002a ). Proposal of Cobetia marina gen. nov., comb. nov., within the family Halomonadaceae, to include the species Halomonas marina . . Syst Appl Microbiol 25, 207–211. [View Article] [PubMed]
    [Google Scholar]
  3. Arahal D. R., Ludwig W., Schleifer K. H., Ventosa A. ( 2002b ). Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. . Int J Syst Evol Microbiol 52, 241–249. [PubMed]
    [Google Scholar]
  4. Arahal D. R., Vreeland R. H., Litchfield C. D., Mormile M. R., Tindall B. J., Oren A., Bejar V., Quesada E., Ventosa A. ( 2007 ). Recommended minimal standards for describing new taxa of the family Halomonadaceae . . Int J Syst Evol Microbiol 57, 2436–2446. [View Article] [PubMed]
    [Google Scholar]
  5. Ben Ali Gam Z., Abdelkafi S., Casalot L., Tholozan J. L., Oueslati R., Labat M. ( 2007 ). Modicisalibacter tunisiensis gen. nov., sp. nov., an aerobic, moderately halophilic bacterium isolated from an oilfield-water injection sample, and emended description of the family Halomonadaceae Franzmann et al. 1989 emend Dobson and Franzmann 1996 emend. Ntougias et al. 2007. . Int J Syst Evol Microbiol 57, 2307–2313. [View Article] [PubMed]
    [Google Scholar]
  6. CLSI ( 2011 ). Performance Standards for Antimicrobial Susceptibility Testing; 21st Informational Supplement M100-S21, vol. 31. Wayne, PA:: Clinical and Laboratory Standards Institute;.
     [Google Scholar]
  7. Cowan S. T., Barrow G. I., Steel K. J., Feltham R. K. A. ( 2003 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria. Cambridge:: Cambridge University Press;.
     [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  9. Dobson S. J., Franzmann P. D. ( 1996 ). Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificana (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae . . Int J Syst Bacteriol 46, 550–558. [View Article]
    [Google Scholar]
  10. Felsenstein J. ( 1985 ). Confidence limits on phylogenics: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  11. Garriga M., Ehrmann M. A., Arnau J., Hugas M., Vogel R. F. ( 1998 ). Carnimonas nigrificans gen. nov., sp. nov., a bacterial causative agent for black spot formation on cured meat products. . Int J Syst Bacteriol 48, 677–686. [View Article] [PubMed]
    [Google Scholar]
  12. Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T. ( 2000 ). Bergey’s Manual of Determinative Bacteriology, vol. 9. Baltimore, MD:: Lippincott Williams & Wilkins;.
     [Google Scholar]
  13. Huß V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  14. Jeong S. H., Lee J. H., Jung J. Y., Lee S. H., Park M. S., Jeon C. O. ( 2013 ). Halomonas cibimaris sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Antonie van Leeuwenhoek 103, 503–512. [View Article] [PubMed]
    [Google Scholar]
  15. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 989–1005. [View Article]
    [Google Scholar]
  16. Kaye J. Z., Márquez M. C., Ventosa A., Baross J. A. ( 2004 ). Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. . Int J Syst Evol Microbiol 54, 499–511. [View Article] [PubMed]
    [Google Scholar]
  17. Kim K. K., Jin L., Yang H. C., Lee S. T. ( 2007 ). Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water. . Int J Syst Evol Microbiol 57, 675–681. [View Article] [PubMed]
    [Google Scholar]
  18. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  19. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  20. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  21. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109–118. [View Article] [PubMed]
    [Google Scholar]
  22. Martínez-Cánovas M. J., Quesada E., Llamas I., Béjar V. ( 2004 ). Halomonas ventosae sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium. . Int J Syst Evol Microbiol 54, 733–737. [View Article] [PubMed]
    [Google Scholar]
  23. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. ( 2002 ). A detailed phenotypic characterisation of the type strains of Halomonas species. . Syst Appl Microbiol 25, 360–375. [View Article] [PubMed]
    [Google Scholar]
  24. Ntougias S., Zervakis G. I., Fasseas C. ( 2007 ). Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996. . Int J Syst Evol Microbiol 57, 1975–1983. [View Article] [PubMed]
    [Google Scholar]
  25. Okamoto T., Taguchi H., Nakamura K., Ikenaga H., Kuraishi H., Yamasato K. ( 1993 ). Zymobacter palmae gen. nov., sp. nov., a new ethanol-fermenting peritrichous bacterium isolated from palm sap. . Arch Microbiol 160, 333–337. [View Article] [PubMed]
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425. [PubMed]
    [Google Scholar]
  27. Sánchez-Porro C., de la Haba R. R., Soto-Ramírez N., Márquez M. C., Montalvo-Rodríguez R., Ventosa A. ( 2009 ). Description of Kushneria aurantia gen. nov., sp. nov., a novel member of the family Halomonadaceae, and a proposal for reclassification of Halomonas marisflavi as Kushneria marisflavi comb. nov., of Halomonas indalinina as Kushneria indalinina comb. nov. and of Halomonas avicenniae as Kushneria avicenniae comb. nov.. Int J Syst Evol Microbiol 59, 397–405. [View Article] [PubMed]
    [Google Scholar]
  28. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  29. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  30. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 1596–1599. [View Article] [PubMed]
    [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  32. Ventosa A., Gutierrez M. C., Garcia M. T., Ruiz-Berraquero F. ( 1989 ). Classification of “Chromobacterium marismortui” in a new genus, Chromohalobacter gen. nov., as Chromohalobacter marismortui comb. nov., nom. rev.. Int J Syst Bacteriol 39, 382–386. [View Article]
    [Google Scholar]
  33. Ventosa A., Nieto J. J., Oren A. ( 1998 ). Biology of moderately halophilic aerobic bacteria. . Microbiol Mol Biol Rev 62, 504–544. [PubMed]
    [Google Scholar]
  34. Vreeland R. H., Litchfield C. D., Martin E. L., Elliot E. ( 1980 ). Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. . Int J Syst Bacteriol 30, 485–495. [View Article]
    [Google Scholar]
  35. Wang Y., Tang S. K., Lou K., Lee J. C., Jeon C. O., Xu L. H., Kim C. J., Li W. J. ( 2009 ). Aidingimonas halophila gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt lake. . Int J Syst Evol Microbiol 59, 3088–3094. [View Article] [PubMed]
    [Google Scholar]
  36. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697–703. [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.056556-0
Loading
Halomonas huangheensis sp. nov., a moderately halophilic bacterium isolated from a saline–alkali soil
Int J Syst Evol Microbiol 64, 915 (2014); https://doi.org/10.1099/ijs.0.056556-0
/content/journal/ijsem/10.1099/ijs.0.056556-0
/content/journal/ijsem/10.1099/ijs.0.056556-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error