1887

Abstract

A novel thermophilic, Gram-stain-positive, facultatively anaerobic, endospore-forming, motile, rod-shaped bacterium, strain C161ab, was isolated from a soil sample collected near Kizildere, Saraykoy-Buharkent power plant in Denizli. The isolate could grow at temperatures between 35 and 70 °C (optimum 55 °C), at pH 6.5–9.0 (optimum pH 8.0–8.5) and with 0–2.5 % NaCl (optimum 0.5 %, w/v). The strain formed cream-coloured, circular colonies and tolerated up to 70 mM boron. Its DNA G+C content was 37.8 mol%. The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. Strain C161ab contained menaquinones MK-7 (96 %) and MK-6 (4 %). The major cellular fatty acids were iso-branched fatty acids: iso-C (52.2 %) and iso-C (28.0 %,) with small amounts of C (7.4 %). Phylogenetic analysis based on the 16S rRNA gene revealed 94.6–96.8 % sequence similarity with all recognized species of the genus . Strain C161ab showed the greatest sequence similarity to DSM 17127 and DSM 17075, both had 96.8 % similarity to strain C161ab, as well as to DSM 15730 (96.6 %). DNA–DNA hybridization revealed low levels of relatedness with the closest relatives of strain C161ab, (21.2 %) and (16.5 %). On the basis of the results obtained from phenotypic, chemotaxonomic, genomic fingerprinting, phylogenetic and hybridization analyses, the isolate is proposed to represent a novel species, sp. nov. (type strain C161ab = DSM 25520 = NCIMB 14851).

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2014-01-01
2019-10-19
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References

  1. Adiguzel A., Ozkan H., Barıs O., Inan K., Gulluce M., Sahin F.. ( 2009;). Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey. . J Microbiol Methods 79:, 321–328. [CrossRef][PubMed]
    [Google Scholar]
  2. Anderson D. G., McKay L. L.. ( 1983;). Simple and rapid method for isolating large plasmid DNA from lactic streptococci. . Appl Environ Microbiol 46:, 549–552.[PubMed]
    [Google Scholar]
  3. Atanassova M., Derekova A., Mandeva R., Sjøholm C., Kambourova M.. ( 2008;). Anoxybacillus bogrovensis sp. nov., a novel thermophilic bacterium isolated from a hot spring in Dolni Bogrov, Bulgaria. . Int J Syst Evol Microbiol 58:, 2359–2362. [CrossRef][PubMed]
    [Google Scholar]
  4. Belduz A. O., Dulger S., Demirbag Z.. ( 2003;). Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium. . Int J Syst Evol Microbiol 53:, 1315–1320. [CrossRef][PubMed]
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef][PubMed]
    [Google Scholar]
  6. Castro-Ochoa L. D., Rodríguez-Gómez C., Valerio-Alfaro G., Oliart Ros R.. ( 2005;). Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. . Enzyme Microb Technol 37:, 648–654. [CrossRef]
    [Google Scholar]
  7. Chen X. G., Stabnikova O., Tay J. H., Wang J. Y., Tay S. T. L.. ( 2004;). Thermoactive extracellular proteases of Geobacillus caldoproteolyticus, sp. nov., from sewage sludge. . Extremophiles 8:, 489–498. [CrossRef][PubMed]
    [Google Scholar]
  8. Cihan A. C.. ( 2013;). Taxonomic classification of Anoxybacillus isolates from geothermal regions in Turkey by 16S rRNA gene sequences and ARDRA, ITS-PCR, Rep-PCR analyses. . Pol J Microbiol 62:, 149–163.[PubMed]
    [Google Scholar]
  9. Cihan A. C., Ozcan B., Cokmus C.. ( 2011;). Anoxybacillus salavatliensis sp. nov., an α-glucosidase producing, thermophilic bacterium isolated from Salavatli, Turkey. . J Basic Microbiol 51:, 136–146. [CrossRef][PubMed]
    [Google Scholar]
  10. Claus D., Berkeley C. W.. ( 1986;). Genus Bacillus Cohn 1872. . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  11. Coleri A., Cokmus C., Ozcan B., Akkoc N., Akcelik M.. ( 2009;). Isolations of α-glucosidase-producing thermophilic bacilli from hot springs of Turkey. . Microbiology (English translation of Mikrobiologiia) 78:, 56–66. [CrossRef]
    [Google Scholar]
  12. Coorevits A., Dinsdale A. E., Halket G., Lebbe L., De Vos P., Van Landschoot A., Logan N. A.. ( 2012;). Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly ‘thermoglucosidasius’); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov.. Int J Syst Evol Microbiol 62:, 1470–1485. [CrossRef][PubMed]
    [Google Scholar]
  13. Daffonchio D., Cherif A., Brusetti L., Rizzi A., Mora D., Boudabous A., Borin S.. ( 2003;). Nature of polymorphisms in 16S-23S rRNA gene intergenic transcribed spacer fingerprinting of Bacillus and related genera. . Appl Environ Microbiol 69:, 5128–5137. [CrossRef][PubMed]
    [Google Scholar]
  14. Dai J., Liu Y., Lei Y., Gao Y., Han F., Xiao Y., Peng H.. ( 2011;). A new subspecies of Anoxybacillus flavithermus ssp. yunnanensis ssp. nov. with very high ethanol tolerance. . FEMS Microbiol Lett 320:, 72–78. [CrossRef][PubMed]
    [Google Scholar]
  15. De Clerck E., Rodríguez-Díaz M., Vanhoutte T., Heyrman J., Logan N. A., De Vos P.. ( 2004;). Anoxybacillus contaminans sp. nov. and Bacillus gelatini sp. nov., isolated from contaminated gelatin batches. . Int J Syst Evol Microbiol 54:, 941–946. [CrossRef][PubMed]
    [Google Scholar]
  16. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  17. Denizci A. A., Kazan D., Abeln E. C. A., Erarslan A.. ( 2004;). Newly isolated Bacillus clausii GMBAE 42: an alkaline protease producer capable to grow under highly alkaline conditions. . J Appl Microbiol 96:, 320–327. [CrossRef][PubMed]
    [Google Scholar]
  18. Derekova A., Sjøholm C., Mandeva R., Kambourova M.. ( 2007;). Anoxybacillus rupiensis sp. nov., a novel thermophilic bacterium isolated from Rupi basin (Bulgaria). . Extremophiles 11:, 577–583. [CrossRef][PubMed]
    [Google Scholar]
  19. Dulger S., Demirbag Z., Belduz A. O.. ( 2004;). Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov.. Int J Syst Evol Microbiol 54:, 1499–1503. [CrossRef][PubMed]
    [Google Scholar]
  20. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  21. Gessesse A., Gashe B. A.. ( 1997;). Production of alkaline protease by an alkaliphilic bacteria isolated from an alkaline soda lake. . Biotechnol Lett 19:, 479–481. [CrossRef]
    [Google Scholar]
  22. Gul-Guven R., Guven K., Poli A., Nicolaus B. J.. ( 2008;). Anoxybacillus kamchatkensis subsp. asaccharedens subsp. nov., a thermophilic bacterium isolated from a hot spring in Batman. . J Gen Appl Microbiol 54:, 327–334. [CrossRef][PubMed]
    [Google Scholar]
  23. Halvorson H.. ( 1966;). α-Glucosidase from yeast. . Methods Enzymol 8:, 559–562. [CrossRef]
    [Google Scholar]
  24. Huss V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  25. Inan K., Belduz A. O., Canakci S.. ( 2013;). Anoxybacillus kaynarcensis sp. nov., a moderately thermophilic, xylanase producing bacterium. . J Basic Microbiol 53:, 410–419. [CrossRef][PubMed]
    [Google Scholar]
  26. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;.
    [Google Scholar]
  27. Kevbrin V. V., Zengler K., Lysenko A. M., Wiegel J.. ( 2005;). Anoxybacillus kamchatkensis sp. nov., a novel thermophilic facultative aerobic bacterium with a broad pH optimum from the Geyser valley, Kamchatka. . Extremophiles 9:, 391–398. [CrossRef][PubMed]
    [Google Scholar]
  28. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  29. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E.. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradorhizobium japonicum. . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  30. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... New York:: Wiley;.
    [Google Scholar]
  31. Lee D. W., Koh Y. S., Kim K. J., Kim B. C., Choi H. J., Kim D. S., Suhartono M. T., Pyun Y.. ( 1999;). Isolation and characterization of a thermophilic lipase from bacillus thermoleovorans ID-1. . FEMS Microbiol Lett 179:, 393–400. [CrossRef][PubMed]
    [Google Scholar]
  32. Mesbah M., Premachandran U., Whitman W.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  33. MIDI ( 2002;). Sherlock Microbial Identification System version 4.5, MIS Operating Manual. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  34. Miller L. T.. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  35. Namsaraev Z. B., Babasanova O. B., Dunaevsky Y. E., Akimov V. N., Barkhutova D. D., Gorlenko V. M., Namsaraev B. B.. ( 2010;). Anoxybacillus mongoliensis sp. nov., a novel thermophilic proteinase producing bacterium isolated from alkaline hot spring, central Mongolia. . Microbiology (English translation of Mikrobiologiia) 79:, 491–499. [CrossRef]
    [Google Scholar]
  36. Pikuta E., Lysenko A., Chuvilskaya N., Mendrock U., Hippe H., Suzina N., Nikitin D., Osipov G., Laurinavichius K.. ( 2000;). Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavitherms comb. nov.. Int J Syst Evol Microbiol 50:, 2109–2117. [CrossRef][PubMed]
    [Google Scholar]
  37. Poli A., Esposito E., Lama L., Orlando P., Nicolaus G., de Appolonia F., Gambacorta A., Nicolaus B.. ( 2006;). Anoxybacillus amylolyticus sp. nov., a thermophilic amylase producing bacterium isolated from Mount Rittmann (Antarctica). . Syst Appl Microbiol 29:, 300–307. [CrossRef][PubMed]
    [Google Scholar]
  38. Poli A., Romano I., Cordella P., Orlando P., Nicolaus B., Ceschi Berrini C.. ( 2009;). Anoxybacillus thermarum sp. nov., a novel thermophilic bacterium isolated from thermal mud in Euganean hot springs, Abano Terme, Italy. . Extremophiles 13:, 867–874. [CrossRef][PubMed]
    [Google Scholar]
  39. Raja C. E., Omine K.. ( 2012;). Characterization of boron tolerant bacteria isolated from a fly ash dumping site for bacterial boron remediation. . Environ Geochem Health 35:, 431–438. [CrossRef]
    [Google Scholar]
  40. Rhuland L. E., Work E., Denman R. F., Hoare D. S.. ( 1955;). The behaviour of the isomers of 2,6-diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77:, 4844–4846. [CrossRef]
    [Google Scholar]
  41. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  42. Schäffer C., Franck W. L., Scheberl A., Kosma P., McDermott T. R., Messner P.. ( 2004;). Classification of isolates from locations in Austria and Yellowstone National Park as Geobacillus tepidamans sp. nov.. Int J Syst Evol Microbiol 54:, 2361–2368. [CrossRef][PubMed]
    [Google Scholar]
  43. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J.. & other authors ( 2002;). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef][PubMed]
    [Google Scholar]
  44. Sung M. H., Kim H., Bae J. W., Rhee S. K., Jeon C. O., Kim K., Kim J. J., Hong S. P., Lee S. G.. & other authors ( 2002;). Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost. . Int J Syst Evol Microbiol 52:, 2251–2255. [CrossRef][PubMed]
    [Google Scholar]
  45. Suzuki Y., Kishigami T., Abe S.. ( 1976;). Production of extracellular α-glucosidase by a thermophilic Bacillus species. . Appl Environ Microbiol 31:, 807–812.[PubMed]
    [Google Scholar]
  46. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  47. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  48. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  49. Versalovic J., Schneider M., De Bruijn F. J., Lupski J. R.. ( 1994;). Genomic fingerprinting of bacteria using repetitive sequence based PCR (rep-PCR). . Methods Mol Cell Biol 5:, 25–40.
    [Google Scholar]
  50. Yumoto I., Hirota K., Kawahara T., Nodasaka Y., Okuyama H., Matsuyama H., Yokota Y., Nakajima K., Hoshino T.. ( 2004;). Anoxybacillus voinovskiensis sp. nov., a moderately thermophilic bacterium from a hot spring in Kamchatka. . Int J Syst Evol Microbiol 54:, 1239–1242. [CrossRef][PubMed]
    [Google Scholar]
  51. Zhang C. M., Huang X. W., Pan W. Z., Zhang J., Wei K. B., Klenk H. P., Tang S. K., Li W. J., Zhang K. Q.. ( 2010;). Anoxybacillus tengchongensis sp. nov. and Anoxybacillus eryuanensis sp. nov., facultatively anaerobic, alkalitolerant bacteria from hot springs. . Int J Syst Evol Microbiol 61:, 118–122. [CrossRef][PubMed]
    [Google Scholar]
  52. Zhang X. Q., Zhang Z. L., Wu N., Zhu X. F., Wu M.. ( 2013;). Anoxybacillus vitaminiphilus sp. nov., a strictly aerobic and moderately thermophilic bacterium isolated from a hot spring. . Int J Syst Evol Microbiol 63:, 4064–4071. [CrossRef][PubMed]
    [Google Scholar]
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