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Volume 64, Issue Pt_1

sp. nov., a thermophilic bacterium isolated from soil near a thermal power plant Free

Abstract

A novel thermophilic, Gram-stain-positive, facultatively anaerobic, endospore-forming, motile, rod-shaped bacterium, strain C161ab, was isolated from a soil sample collected near Kizildere, Saraykoy-Buharkent power plant in Denizli. The isolate could grow at temperatures between 35 and 70 °C (optimum 55 °C), at pH 6.5–9.0 (optimum pH 8.0–8.5) and with 0–2.5 % NaCl (optimum 0.5 %, w/v). The strain formed cream-coloured, circular colonies and tolerated up to 70 mM boron. Its DNA G+C content was 37.8 mol%. The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. Strain C161ab contained menaquinones MK-7 (96 %) and MK-6 (4 %). The major cellular fatty acids were iso-branched fatty acids: iso-C (52.2 %) and iso-C (28.0 %,) with small amounts of C (7.4 %). Phylogenetic analysis based on the 16S rRNA gene revealed 94.6–96.8 % sequence similarity with all recognized species of the genus . Strain C161ab showed the greatest sequence similarity to DSM 17127 and DSM 17075, both had 96.8 % similarity to strain C161ab, as well as to DSM 15730 (96.6 %). DNA–DNA hybridization revealed low levels of relatedness with the closest relatives of strain C161ab, (21.2 %) and (16.5 %). On the basis of the results obtained from phenotypic, chemotaxonomic, genomic fingerprinting, phylogenetic and hybridization analyses, the isolate is proposed to represent a novel species, sp. nov. (type strain C161ab = DSM 25520 = NCIMB 14851).

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Funding
This study was supported by the:
  • Scientific Research Project Office of Ankara University (Award 11B4240003)
© 2014 IUMS

Erratum

An erratum has been published for this content:
sp. nov., a thermophilic bacterium isolated from soil near a thermal power plant

Erratum

An erratum has been published for this content:
sp. nov., a thermophilic bacterium isolated from soil near a thermal power plant
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2014-01-01
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Anoxybacillus calidus sp. nov., a thermophilic bacterium isolated from soil near a thermal power plant
Int J Syst Evol Microbiol 64, 211 (2014); https://doi.org/10.1099/ijs.0.056549-0
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