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Abstract

An aerobic, Gram-stain negative, pale, rod-shaped, nitrogen-fixing bacterial strain, DDT-3, was isolated from dichlorodiphenyltrichloroethane-contaminated soil in Liyang, PR China. Strain DDT-3 grew at temperatures ranging from 20 to 40 °C (optimum 30–37 °C) and a pH of between 5.0 and 10.0 (optimum pH 7.0–8.0). The G+C content of the total DNA was 70.1 mol%. The 16S rRNA gene sequence of strain DDT-3 showed the highest similarity to that of CC4 (99.6 %), followed by B6-8 (93.3 %), 5YN9-8 (93.0 %) and YIM D10 (93.0 %). Strain DDT-3 showed less than 92.6 % similarity with other species of the family . The major cellular fatty acids of strain DDT-3 were C cyclo ω8 (42.6 %), C (33.2 %) and Cω7 (10.0 %). The only respiratory quinone was ubiquinone Q-10. The characteristic diamino acid of the peptidoglycan was -diaminopimelic acid. The major polar lipids were glycolipid, lipid, phosphatidylcholine, aminolipid, phosphatidylmethylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The polyamine profile consisted of major amounts of putrescine (92.9 %) and minor amounts of spermidine (5.0 %) and spermine (2.1 %). These chemotaxonomic data support the affiliation of strain DDT-3 with the genus . The DNA–DNA hybridization value between strain DDT-3 and strain CC4 was 47.8 % (reciprocal 44.3 %). DNA–DNA hybridization data as well as the biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain DDT-3 and strain CC4. Strain DDT-3, therefore, represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DDT-3 ( = KACC 16601 = CCTCC AB 2013167).

Funding
This study was supported by the:
  • , Outstanding Youth Foundation of Jiangsu Province , (Award BK20130029)
  • , Program for New Century Excellent Talents in University , (Award NCET-12-0892)
  • , Fundamental Research Funds for the Central Universities , (Award KYZ201422)
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2014-10-01
2021-03-06
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